TY - JOUR
T1 - Role of protein kinase A in regulating steroid sulfate uptake for estrogen production in human placental choriocarcinoma cells
AU - Tomi, M.
AU - Miyata, Y.
AU - Noguchi, S.
AU - Nishimura, S.
AU - Nishimura, T.
AU - Nakashima, E.
N1 - Funding Information:
This work was supported in part by JSPS KAKENHI Grant Numbers 21890246 , 23790199 , 25560063 , 25860396 . This work was also supported in part by the MEXT-Supported Program for Strategic Research at Private Universities, the Food Safety Commission of Japan (Cabinet Office) ( #1107 ), Takeda Science Foundation , and The Nakatomi Foundation .
PY - 2014/8
Y1 - 2014/8
N2 - The purpose of this study is to assess the role of the protein kinase A (PKA) in regulating uptake of dehydroepiandrosterone sulfate (DHEAS), an estrogen precursor, by syncytiotrophoblasts. Forskolin, a PKA activator, significantly increased [3H]DHEAS uptake and the mRNA expression levels of organic anion transporter (OAT) 4 and CYP19A1 in choriocarcinoma JEG-3 cells, while other steroid sulfate transporters present in the placenta showed no change in expression level. KT5720, a PKA inhibitor, attenuated these effects of forskolin. Accordingly, the PKA pathway appears to play an important role in estrogen synthesis by cooperatively regulating OAT4 and steroidogenic enzymes in syncytiotrophoblasts.
AB - The purpose of this study is to assess the role of the protein kinase A (PKA) in regulating uptake of dehydroepiandrosterone sulfate (DHEAS), an estrogen precursor, by syncytiotrophoblasts. Forskolin, a PKA activator, significantly increased [3H]DHEAS uptake and the mRNA expression levels of organic anion transporter (OAT) 4 and CYP19A1 in choriocarcinoma JEG-3 cells, while other steroid sulfate transporters present in the placenta showed no change in expression level. KT5720, a PKA inhibitor, attenuated these effects of forskolin. Accordingly, the PKA pathway appears to play an important role in estrogen synthesis by cooperatively regulating OAT4 and steroidogenic enzymes in syncytiotrophoblasts.
KW - Dehydroepiandrosterone sulfate
KW - Estrogen synthesis
KW - OAT4
KW - Protein kinase A
KW - Syncytiotrophoblast
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U2 - 10.1016/j.placenta.2014.06.003
DO - 10.1016/j.placenta.2014.06.003
M3 - Article
C2 - 24969759
AN - SCOPUS:84905399233
SN - 0143-4004
VL - 35
SP - 658
EP - 660
JO - Placenta
JF - Placenta
IS - 8
ER -