Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line

Makoto Noda; Minoru Ko; Akihiko Ogura; Ding Gan Liu; Takehiko Amano; Toshiya Takano; Yoji Ikawa

doi:10.1038/318073a0

Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line

Makoto Noda, Minoru Ko, Akihiko Ogura, Ding Gan Liu, Takehiko Amano, Toshiya Takano, Yoji Ikawa

The Sakaguchi Laboratory of Developmental Biology Department of Systems Medicine

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Abstract

The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha7-MSV) on various types of cells in vitro are well documented^1-8. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones^9,10. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.

Original language	English
Pages (from-to)	73-75
Number of pages	3
Journal	Nature
Volume	318
Issue number	6041
DOIs	https://doi.org/10.1038/318073a0
Publication status	Published - 1985

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title = "Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line",

abstract = "The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha7-MSV) on various types of cells in vitro are well documented1-8. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones9,10. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.",

author = "Makoto Noda and Minoru Ko and Akihiko Ogura and Liu, {Ding Gan} and Takehiko Amano and Toshiya Takano and Yoji Ikawa",

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T1 - Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line

AU - Noda, Makoto

AU - Ko, Minoru

AU - Ogura, Akihiko

AU - Liu, Ding Gan

AU - Amano, Takehiko

AU - Takano, Toshiya

AU - Ikawa, Yoji

PY - 1985

Y1 - 1985

N2 - The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha7-MSV) on various types of cells in vitro are well documented1-8. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones9,10. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.

AB - The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha7-MSV) on various types of cells in vitro are well documented1-8. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones9,10. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.

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Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line

Abstract

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