Selection of peptides with the recognition of stereoregular PMMA from phage library

Toshiki Sawada; Hisao Matsuno; Teruhiko Matsubara; Toshinori Sato; Tatsuki Kitayama; Takeshi Serizawa

Selection of peptides with the recognition of stereoregular PMMA from phage library

Toshiki Sawada, Hisao Matsuno, Teruhiko Matsubara, Toshinori Sato, Tatsuki Kitayama, Takeshi Serizawa

Department of Biosciences and Informatics

Research output: Contribution to conference › Paper › peer-review

Abstract

To identify peptides with affinity selective for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), phage library displaying a heptamer random peptide was used. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. Binding affinity and specificity of these phage clones were quantitatively investigated by enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Other

Other	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

Peptide
Phage library
Poly(methyl methacrylate)
Tacticity

ASJC Scopus subject areas

General Engineering

Cite this

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title = "Selection of peptides with the recognition of stereoregular PMMA from phage library",

abstract = "To identify peptides with affinity selective for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), phage library displaying a heptamer random peptide was used. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. Binding affinity and specificity of these phage clones were quantitatively investigated by enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.",

keywords = "Peptide, Phage library, Poly(methyl methacrylate), Tacticity",

author = "Toshiki Sawada and Hisao Matsuno and Teruhiko Matsubara and Toshinori Sato and Tatsuki Kitayama and Takeshi Serizawa",

year = "2005",

month = dec,

day = "1",

language = "English",

note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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TY - CONF

T1 - Selection of peptides with the recognition of stereoregular PMMA from phage library

AU - Sawada, Toshiki

AU - Matsuno, Hisao

AU - Matsubara, Teruhiko

AU - Sato, Toshinori

AU - Kitayama, Tatsuki

AU - Serizawa, Takeshi

PY - 2005/12/1

Y1 - 2005/12/1

N2 - To identify peptides with affinity selective for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), phage library displaying a heptamer random peptide was used. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. Binding affinity and specificity of these phage clones were quantitatively investigated by enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

AB - To identify peptides with affinity selective for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), phage library displaying a heptamer random peptide was used. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. Binding affinity and specificity of these phage clones were quantitatively investigated by enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

KW - Peptide

KW - Phage library

KW - Poly(methyl methacrylate)

KW - Tacticity

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M3 - Paper

AN - SCOPUS:33645650884

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Selection of peptides with the recognition of stereoregular PMMA from phage library

Abstract

Other

Keywords

ASJC Scopus subject areas

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