Separation of catechins by temperature-responsive chromatography

Chikako Sakamoto, Yuji Okada, Hideko Kanazawa, Akihiko Kikuchi, Teruo Okano

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


We have developed a new method of chromatography, temperature-responsive chromatography, using packing materials modified with poly(N- isopropylacrylamide) (PNIPAAm). The temperature-responsive polymer, PNIPAAm, shows a reversible and sharp phase transition in an aqueous solution. The PNIPAAm-modified stationary phase alters the properties of the surface from hydrophilic to hydrophobic in response to temperature changes at the phase-transition temperature. In this study, we examined the separation of five catechins (epigallocatechin, epicatechin, catechin, epigallocatechin gallate and epicatechin gallate) using a PNIPAAm-modified column without changing the composition of the mobile phase. In this method, interactions between catechins and the stationary phases were controlled by the temperature. The retention times of all catechins were retarded, because the interaction increased at elevated temperature. We achieved the separation of catechins using only an aqueous solution as a mobile phase by temperature-responsive chromatography.

Original languageEnglish
Pages (from-to)903-906
Number of pages4
Issue number10
Publication statusPublished - 2003 Oct
Externally publishedYes


  • Catechins
  • Poly(N-isopropylacrylamide)
  • Short column
  • Temperature-responsive polymer

ASJC Scopus subject areas

  • Analytical Chemistry


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