Sequential insertion of multiple I-SceI recognition sites at designed loci of the bacillus subtilis 168 genome

Mitsuhiro Itaya

doi:10.1271/bbb.110528

Sequential insertion of multiple I-SceI recognition sites at designed loci of the bacillus subtilis 168 genome

Mitsuhiro Itaya

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives.

Original language	English
Pages (from-to)	180-182
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	76
Issue number	1
DOIs	https://doi.org/10.1271/bbb.110528
Publication status	Published - 2012
Externally published	Yes

Keywords

Bacillus subtilis
Genome structure
Genome vector
Rare cutting enzymes

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.110528

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abstract = "Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives.",

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KW - Genome structure

KW - Genome vector

KW - Rare cutting enzymes

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Sequential insertion of multiple I-SceI recognition sites at designed loci of the bacillus subtilis 168 genome

Abstract

Keywords

ASJC Scopus subject areas

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