TY - JOUR
T1 - Serotonin-induced nitric oxide production in the ventral nerve cord of the earthworm, Eisenia fetida
AU - Kitamura, Yoshiichiro
AU - Naganoma, Yuichi
AU - Horita, Haruhito
AU - Ogawa, Hiroto
AU - Oka, Kotaro
N1 - Funding Information:
We thank Dr Kazuo Tanishita, Department of System Design Engineering, Faculty of Science and Technology, Keio University, for lending us the microscope for observation of NADPH-d histochemical preparations. This work was partially supported by the ‘Research for the Future’ Program of the Japan Society for the Promotion of Science (contract number JSPS-RFTF 96100102), and by the Academic Frontier Promotion Project ‘Science and Technology Program on Molecule, Supra-Molecules and Supra-Structured Materials’ of the Japan Ministry of Education, Culture, Sports, Science and Technology.
PY - 2001
Y1 - 2001
N2 - Effect of serotonin on nitric oxide (NO) production in the ventral nerve cord (VNC) of the earthworm Eisenia fetida was investigated by a bio-imaging and an electrochemical technique. In the bio-imaging, the spatial pattern of NO production in VNC was visualized using an NO-specific fluorescent dye, diaminofluorescein-2 diacethyl (DAF-2 DA). Application of serotonin (100 μM) increased NO production in VNC by about 65% (P<0.05), compared with basal NO production. The increase was mainly from the nitergic neurons in the ventral side of VNC. In the electrochemical technique, real-time basal and serotonin-induced NO production was estimated with an NO-specific electrode. On the ventral surface of VNC, the estimated basal NO production was stable at 200±52 nM, and was transiently augmented to 840±193 nM by the addition of 10 μM serotonin. In conclusion, the estimated basal NO production in the earthworm VNC is relatively high compared with other nervous systems earlier reported, and transiently augmented by serotonin. Our results suggest that NO signaling in VNC is involved in neuromodulation by serotonin.
AB - Effect of serotonin on nitric oxide (NO) production in the ventral nerve cord (VNC) of the earthworm Eisenia fetida was investigated by a bio-imaging and an electrochemical technique. In the bio-imaging, the spatial pattern of NO production in VNC was visualized using an NO-specific fluorescent dye, diaminofluorescein-2 diacethyl (DAF-2 DA). Application of serotonin (100 μM) increased NO production in VNC by about 65% (P<0.05), compared with basal NO production. The increase was mainly from the nitergic neurons in the ventral side of VNC. In the electrochemical technique, real-time basal and serotonin-induced NO production was estimated with an NO-specific electrode. On the ventral surface of VNC, the estimated basal NO production was stable at 200±52 nM, and was transiently augmented to 840±193 nM by the addition of 10 μM serotonin. In conclusion, the estimated basal NO production in the earthworm VNC is relatively high compared with other nervous systems earlier reported, and transiently augmented by serotonin. Our results suggest that NO signaling in VNC is involved in neuromodulation by serotonin.
KW - Diaminofluorescein-2 diacethyl (DAF-2 DA)
KW - Earthworm
KW - Electrochemistry
KW - Imaging
KW - NO electrode
KW - Nitric oxide (NO)
KW - Serotonin
KW - Ventral nerve cord (VNC)
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U2 - 10.1016/S0168-0102(01)00271-1
DO - 10.1016/S0168-0102(01)00271-1
M3 - Article
C2 - 11591440
AN - SCOPUS:0034809001
SN - 0168-0102
VL - 41
SP - 129
EP - 134
JO - Neuroscience Research
JF - Neuroscience Research
IS - 2
ER -