Serum immunoglobulin G Fc region N-glycosylation profiling by matrix-assisted laser desorption/ionization mass spectrometry can distinguish breast cancer patients from cancer-free controls

Nobuko Kawaguchi-Sakita, Kaoru Kaneshiro-Nakagawa, Masahiro Kawashima, Masahiro Sugimoto, Mariko Tokiwa, Eiji Suzuki, Shigeki Kajihara, Yuichiro Fujita, Shinichi Iwamoto, Koichi Tanaka, Masakazu Toi

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Herein, we report that breast cancer (BC) patients can be distinguished from cancer-free (NC) controls by serum immunoglobulin G (IgG) crystallizable fragment (Fc) region N-glycosylation profiling using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Recently, there has been much progress in the field of tumor immunology. However, to date, the role and biomarker potential of IgG Fc region N-glycosylation, which affects the function of antibodies, have not been examined in BC. In the present study, we profiled serum IgG Fc region N-glycans in BC patients (N = 90) and NC controls (N = 54) using MALDI-MS. An IgG Fc region N-glycan-based multiple logistic regression model was produced which could distinguish BC patients from NC controls (area under the receiver operative characteristic curve = 0.874). Furthermore, stage 0 patients could also be distinguished using this model. These results suggest that an unknown humoral factor or soluble mediator affects IgGs from the earliest stage of breast cancer, and also suggests that IgG Fc region N-glycosylation may play a role in tumor biology. Although further investigation is required, our findings are the evidence that IgG N-glycan profiling has the potential to be used as a breast cancer biomarker and may provide the insights into tumor immunology.

Original languageEnglish
Pages (from-to)1140-1145
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume469
Issue number4
DOIs
Publication statusPublished - 2016 Jan 22

Keywords

  • Biomarker
  • Breast cancer
  • IgG Fc region N-glycosylation
  • Tumor immunity

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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