Serum metabolomic profiling of patients with non-infectious uveitis

Hiroyuki Shimizu, Yoshihiko Usui, Masaki Asakage, Naoya Nezu, Ryo Wakita, Kinya Tsubota, Masahiro Sugimoto, Hiroshi Goto

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The activities of various metabolic pathways can influence the pathogeneses of autoimmune diseases, and intrinsic metabolites can potentially be used to diagnose diseases. However, the metabolomic analysis of patients with uveitis has not yet been conducted. Here, we profiled the serum metabolomes of patients with three major forms of uveitis (Behҫet’s disease (BD), sarcoidosis, and Vogt-Koyanagi-Harada disease (VKH)) to identify potential biomarkers. This study included 19 BD, 20 sarcoidosis, and 15 VKH patients alongside 16 healthy control subjects. The metabolite concentrations in their sera were quantified using liquid chromatography with time-of-flight mass spectrometry. The discriminative abilities of quantified metabolites were evaluated by four comparisons: control vs. three diseases, and each disease vs. the other two diseases (such as sarcoidosis vs. BD + VKH). Among 78 quantified metabolites, 24 kinds of metabolites showed significant differences in these comparisons. Four multiple logistic regression models were developed and validated. The area under the receiver operating characteristic (ROC) curve (AUC) in the model to discriminate disease groups from control was 0.72. The AUC of the other models to discriminate sarcoidosis, BD, and VKH from the other two diseases were 0.84, 0.83, and 0.73, respectively. This study provides potential diagnostic abilities of sarcoidosis, BD, and VKH using routinely available serum samples that can be collected with minimal invasiveness.

Original languageEnglish
Article number3955
Pages (from-to)1-10
Number of pages10
JournalJournal of Clinical Medicine
Volume9
Issue number12
DOIs
Publication statusPublished - 2020 Dec
Externally publishedYes

Keywords

  • Behҫet’s disease
  • Biomarker
  • Liquid chromatography-mass spectrometry
  • Metabolomics
  • Sarcoidosis
  • Serum
  • Vogt-Koyanagi-Harada disease

ASJC Scopus subject areas

  • General Medicine

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