Sex-specific histone modifications in mouse fetal and neonatal germ cells

Yukiko Kawabata, Asuka Kamio, Yuko Jincho, Akihiko Sakashita, Tomoya Takashima, Hisato Kobayashi, Yasuhisa Matsui, Tomohiro Kono

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Aims: Epigenetic signatures of germline cells are dynamically reprogrammed to induce appropriate differentiation, development and sex specification. We investigated sex-specific epigenetic changes in mouse fetal germ cells (FGCs) and neonatal germ cells. Materials & methods: Six histone marks in mouse E13.5 FGCs and P1 neonatal germ cells were analyzed by chromatin immunoprecipitation and sequencing. These datasets were compared with transposase-accessible chromatin sites, DNA methylation and transcriptome. Results: Different patterns of each histone mark were detected in female and male FGCs, and H3K4me3/H3K27me3 bivalent marks were enriched in different chromosomal regions of female and male FGCs. Conclusion: Our results suggest that histone modifications may affect FGC gene expression following DNA methylation erasure, contributing to the differentiation into female and male germ cells.

Original languageEnglish
Pages (from-to)543-561
Number of pages19
Issue number5
Publication statusPublished - 2019 Apr
Externally publishedYes


  • ATAC-seq
  • DNA methylation
  • epigenetics
  • fetal germ cells
  • gene expression
  • histone modifications
  • sex differences
  • transcriptome

ASJC Scopus subject areas

  • Genetics
  • Cancer Research


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