Shear stress attenuates endothelin and endothelin-converting enzyme expression through oxidative stress

Ken Masatsugu, Hiroshi Itoh, Tae Haw Chun, Takatoshi Saito, Jun Yamashita, Kentaro Doi, Mayumi Inoue, Naoki Sawada, Yasutomo Fukunaga, Satsuki Sakaguchi, Masakatsu Sone, Kenichi Yamahara, Takami Yurugi, Kazuwa Nakao

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


Shear stress is known to dilate blood vessels and exert an antiproliferative effect on vascular walls. These effects have partly been ascribed to shear stress-induced regulation of the secretion of endothelium-derived vasoactive substances. In this study, to elucidate the role of shear stress in endothelin production by endothelial cells, we examined the effect of physiological shear stress on the mRNA expression of endothelin-converting enzyme-1 (ECE-1) as well as endothelin-1 (ET-1) in cultured bovine carotid artery endothelial cells (BAECs) and human umbilical vein endothelial cells (HUVECs), using a parallel plate-type flow chamber. ECE-1 mRNA expression was significantly down-regulated by shear stress in an intensity- and time-dependent manner within the physiological range (1.5 to 15 dyn/cm2). ET-1 mRNA expression decreased together with ECE-1 mRNA expression. Shear stress at 15 dyn/cm2 for 30 min induced a significant increase in the intracellular peroxide concentration, and the down-regulation of ECE-1 and ET-1 mRNA expression by shear stress was attenuated almost completely on treatment with N-acetyl cysteine (NAC), an antioxidant (20 mM). Furthermore, when H2O2 (0.5 to 2 mM) was added to BAECs in static culture, the ECE-1 as well as ET-1 mRNA expression was attenuated in proportion to the concentration of H2O2. It is suggested that endothelial cells sense shear stress as oxidative stress and transduce signal for the regulation of the gene expression of ECE as well as ET to attenuate vascular tone and inhibit the proliferation of vascular smooth muscle cells.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalRegulatory Peptides
Issue number1-3
Publication statusPublished - 2003 Mar 28
Externally publishedYes


  • Angiotensin-converting enzyme
  • Antioxidant
  • Endothelial cells
  • Flow
  • Hydrogen peroxide
  • mRNA

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience


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