TY - JOUR
T1 - Shear-stress effect on mitochondrial membrane potential and albumin uptake in cultured endothelial cells
AU - Kudo, Susumu
AU - Morigaki, Ryoma
AU - Saito, Junichi
AU - Ikeda, Mariko
AU - Oka, Kotaro
AU - Tanishita, Kazuo
N1 - Funding Information:
This study was partially supported by a Grant-in-Aid for General Scientific Research (08455095) from Japan’s Ministry of Education, Science and Culture, the Science Research Promotion Fund in Japan, by a Grant-in-Aid for Encouragement of Young Scientists (11780629) from the Japan Society for the Promotion of Science, and by a Keio University Special Grant-in-Aid Innovative Corroborative Research Project.
PY - 2000/4/13
Y1 - 2000/4/13
N2 - Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow in vivo and shear stress affects ATP-dependent macromolecular transport in ECs. However, the relationship between the ATP production and shear stress is still unclear. We, therefore, evaluated mitochondrial ATP synthesis activity in cultured endothelial cells exposed to shear stress, using a confocal laser scanning microscope (CLSM) and a mitochondrial membrane potential probe (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolycarbocyanine iodide, JC-1). Low shear stress (10 dyn/cm2) increased mitochondrial membrane potential by 30%. On the contrary, high shear stress (60 dyn/cm2) decreased it by 20%. This observation was consistent with the ATP-dependent albumin uptake into endothelial cells. Our results indicate that ATP synthetic activity is related to the albumin uptake into endothelial cells. (C) 2000 Academic Press.
AB - Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow in vivo and shear stress affects ATP-dependent macromolecular transport in ECs. However, the relationship between the ATP production and shear stress is still unclear. We, therefore, evaluated mitochondrial ATP synthesis activity in cultured endothelial cells exposed to shear stress, using a confocal laser scanning microscope (CLSM) and a mitochondrial membrane potential probe (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolycarbocyanine iodide, JC-1). Low shear stress (10 dyn/cm2) increased mitochondrial membrane potential by 30%. On the contrary, high shear stress (60 dyn/cm2) decreased it by 20%. This observation was consistent with the ATP-dependent albumin uptake into endothelial cells. Our results indicate that ATP synthetic activity is related to the albumin uptake into endothelial cells. (C) 2000 Academic Press.
KW - ATP
KW - Endothelial cell
KW - Macromolecule uptake
KW - Mitochondrial membrane potential
KW - Shear stress
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U2 - 10.1006/bbrc.2000.2482
DO - 10.1006/bbrc.2000.2482
M3 - Article
C2 - 10753672
AN - SCOPUS:0034643295
SN - 0006-291X
VL - 270
SP - 616
EP - 621
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -