Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction

M. Fukuyama, K. Kawakami, K. Sakai, I. Itagaki, K. Kawano, M. Handa, Y. Ikeda

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2 Citations (Scopus)


To investigate the interaction between platelets and tumor cells under well-defined flow conditions, the effect of tumor cells on platelet aggregation induced by shear stress was studied using a cone and plate viscometer adapted for measuring transmitted light intensity. Aggregation was markedly enhanced by HMV-1 cells in a cell number-dependent fashion under shear stress of 12 dyne/cm2. Enhancement was not observed at a high shear stress of 108 dyne/cm2. A monoclonal antibody against GPIIb/IIIa, 7E3 completely abolished enhancement of aggregation by HMV-1. Apyrase had similar inhibitory effects. Scanning electronmicroscopy showed that direct contacts of platelets with HMV-1 cells could be demonstrated when platelet-platelet interaction was inhibited by 7E3 or apyrase. These results may indicate that, at a shear stress of 12 dyne/cm2, direct contacts of platelets and HMV-1 cells may trigger enhancement of platelet aggregation.

Original languageEnglish
Pages (from-to)M189-M191
JournalASAIO Transactions
Issue number3
Publication statusPublished - 1990 Jul 1

ASJC Scopus subject areas

  • Biophysics


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