Simple and Rapid Staining for Detection of Entamoeba Cysts and Other Protozoans with Fluorochromes

Fumihiko Kawamoto, Sahoko Mizuno, Hisashi Fujioka, Nobuo Kumada, Etsuro Sugiyama, Tsutomu Takeuchi, Seiki Kobayashi, Motohiro Iseki, Minoru Yamada, Yoshitsugu Matsumoto, Tatsuya Tegoshi, Yukio Yoshida

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13 Citations (Scopus)


Three fluorochromes were applied to stain various parasitic protozoans. By double staining with 4′,6-diamidino-2-phenylindole and propidium iodide, differentiation of the nuclei from the cytoplasm can easily be achieved within several seconds. The chromatoid bodies in Entamoeba cysts were stained bright red. Plasmodium yoelii at all stages except late trophozoites and young gametocytes was easily identified. In the oocysts of Cryptosporidium sp., the nuclei and cytoplasm of the sporozoites fluoresced bluish white and red, respectively, whereas the residual body appeared blue or green. The third fluorochrome, Calcofluor white M2R, was suitable for detecting the cysts of Entamoeba spp. and Chilomastix mesnili.

Original languageEnglish
Pages (from-to)35-46
Number of pages12
JournalJapanese Journal of Medical Science and Biology
Issue number1
Publication statusPublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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