Abstract
An analytical method for organic acids (formic acid, fumaric acid, tartaric acid, malic acid, citric acid, acetic acid, lactic acid and propionic acid) in formula feeds by capillary electrophoresis (CE) was established. Organic acids were simultaneously extracted with water. The extract was filtered, diluted with 0.02 mol/L NaOH, and deproteinized by an ultrafiltration filter (30 kDa cut-off). The recovery test was conducted with 3 kinds of formula feed spiked with 8 kinds of organic acids at levels of 0.05~2.0%. The mean recovery values were in the range of 85.1~107.3%, and the relative standard deviation of repeatability (RSD) was within 13.6%. A collaborative study (3 laboratories) was conducted with formula feed spiked with 8 kinds of organic acids at levels of 0.1~1.0%. The mean recovery was 87.3~105.0%, and the relative standard deviation of reproducibility (RSD(R)) was within 16.3%.
| Original language | English |
|---|---|
| Pages (from-to) | 261-267 |
| Number of pages | 7 |
| Journal | Journal of the Food Hygienic Society of Japan |
| Volume | 41 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2000 Jan 1 |
| Externally published | Yes |
Keywords
- Capillary electrophoresis
- Collaborative study
- Feed additive
- Formula feed
- Organic acid
- Ultrafiltration filter
ASJC Scopus subject areas
- Food Science
- Public Health, Environmental and Occupational Health
