TY - GEN
T1 - Simultaneous measurement of blood flow distribution and oxygen tension by photo-excitation in organ microcirculation
AU - Tsukada, Kosuke
AU - Hase, Kentaro
AU - Sekizuka, Eiichi
AU - Oshio, Chikara
AU - Minamitani, Haruyuki
N1 - Publisher Copyright:
© 2000 IEEE.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - The microcirculatory system plays a very important role in delivering oxygen and materials to tissues and organs. In this study, we set up a new system with two light sources to measure blood flow velocity, vessel diameter, and pO2 simultaneously in organ microcirculation. Pd-meso-tetra(4-carboxyphenyl)-porphyrin was employed as an oxygen-sensitive probe, and was excited with the second harmonic of a Nd:YAG pulse laser (wavelength: 532 nm) at 1 Hz. The phosphorescence lifetime was obtained from the emission decay curve and pO2 was calculated from the Stern-Volmer equation. At the same time, blood flow in organ microvesscls was visualized by perfusing red blood cells labeled with fluorescent isothiocyanate. Blood flow velocity and vessel diameter were quantified by processing images with a personal computer. Through measurements on rats in vivo, the changes in blood flow velocity and pO2 in organ microvessels were quantified during asphyxia and hemorrhagic shock. The results demonstrate that the system can measure blood flow and oxygen delivery in the microcirculatory system. In the future, we will use this system to analyze pathologic physiology, such as blood flow change in diabetes mellitus.
AB - The microcirculatory system plays a very important role in delivering oxygen and materials to tissues and organs. In this study, we set up a new system with two light sources to measure blood flow velocity, vessel diameter, and pO2 simultaneously in organ microcirculation. Pd-meso-tetra(4-carboxyphenyl)-porphyrin was employed as an oxygen-sensitive probe, and was excited with the second harmonic of a Nd:YAG pulse laser (wavelength: 532 nm) at 1 Hz. The phosphorescence lifetime was obtained from the emission decay curve and pO2 was calculated from the Stern-Volmer equation. At the same time, blood flow in organ microvesscls was visualized by perfusing red blood cells labeled with fluorescent isothiocyanate. Blood flow velocity and vessel diameter were quantified by processing images with a personal computer. Through measurements on rats in vivo, the changes in blood flow velocity and pO2 in organ microvessels were quantified during asphyxia and hemorrhagic shock. The results demonstrate that the system can measure blood flow and oxygen delivery in the microcirculatory system. In the future, we will use this system to analyze pathologic physiology, such as blood flow change in diabetes mellitus.
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U2 - 10.1109/MMB.2000.893747
DO - 10.1109/MMB.2000.893747
M3 - Conference contribution
AN - SCOPUS:84952673859
T3 - 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
SP - 84
EP - 87
BT - 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
A2 - Dittmar, Andre
A2 - Beebe, David
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, MMB 2000
Y2 - 12 October 2000 through 14 October 2000
ER -
