Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential

Matti Lam; Tsukasa Sanosaka; Anders Lundin; Kent Imaizumi; Damla Etal; Fredrik H. Karlsson; Maryam Clausen; Jonathan Cairns; Ryan Hicks; Jun Kohyama; Malin Kele; Hideyuki Okano; Anna Falk

doi:10.1111/gtc.12731

Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential

Matti Lam, Tsukasa Sanosaka, Anders Lundin, Kent Imaizumi, Damla Etal, Fredrik H. Karlsson, Maryam Clausen, Jonathan Cairns, Ryan Hicks, Jun Kohyama, Malin Kele, Hideyuki Okano, Anna Falk

Department of Physiology

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.

Original language	English
Pages (from-to)	836-847
Number of pages	12
Journal	Genes to Cells
Volume	24
Issue number	12
DOIs	https://doi.org/10.1111/gtc.12731
Publication status	Published - 2019 Dec 1

Keywords

glia
induced pluripotent stem cells
modeling gliogenesis
modeling neurogenesis
neural stem cells
neurons
single cell RNA sequencing

ASJC Scopus subject areas

Genetics
Cell Biology

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10.1111/gtc.12731

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Lam, M., Sanosaka, T., Lundin, A., Imaizumi, K., Etal, D., Karlsson, F. H., Clausen, M., Cairns, J., Hicks, R., Kohyama, J., Kele, M., Okano, H., & Falk, A. (2019). Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential. Genes to Cells, 24(12), 836-847. https://doi.org/10.1111/gtc.12731

Lam, M, Sanosaka, T, Lundin, A, Imaizumi, K, Etal, D, Karlsson, FH, Clausen, M, Cairns, J, Hicks, R, Kohyama, J, Kele, M, Okano, H & Falk, A 2019, 'Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential', Genes to Cells, vol. 24, no. 12, pp. 836-847. https://doi.org/10.1111/gtc.12731

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abstract = "We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.",

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author = "Matti Lam and Tsukasa Sanosaka and Anders Lundin and Kent Imaizumi and Damla Etal and Karlsson, {Fredrik H.} and Maryam Clausen and Jonathan Cairns and Ryan Hicks and Jun Kohyama and Malin Kele and Hideyuki Okano and Anna Falk",

note = "Funding Information: Authors thank the iPS Core (ipscore.se) and ESCG (escg.se) facilities at Karolinska Institute. Anders Lundin is part of AstraZeneca's Post-Doc Program. Anders Lundin, Damla Etal, Fredrik H. Karlsson, Maryam Clausen and Jonathan Cairns are employees of AstraZeneca. Publisher Copyright: {\textcopyright} 2019 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd",

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AU - Lam, Matti

AU - Sanosaka, Tsukasa

AU - Lundin, Anders

AU - Imaizumi, Kent

AU - Etal, Damla

AU - Karlsson, Fredrik H.

AU - Clausen, Maryam

AU - Cairns, Jonathan

AU - Hicks, Ryan

AU - Kohyama, Jun

AU - Kele, Malin

AU - Okano, Hideyuki

AU - Falk, Anna

N1 - Funding Information: Authors thank the iPS Core (ipscore.se) and ESCG (escg.se) facilities at Karolinska Institute. Anders Lundin is part of AstraZeneca's Post-Doc Program. Anders Lundin, Damla Etal, Fredrik H. Karlsson, Maryam Clausen and Jonathan Cairns are employees of AstraZeneca. Publisher Copyright: © 2019 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd

PY - 2019/12/1

Y1 - 2019/12/1

N2 - We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.

AB - We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.

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Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential

Abstract

Keywords

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