TY - GEN
T1 - Single neuron observation in a 3D neuronal tissue blook
AU - Kato-Negishi, Midori
AU - Onoe, Hiroaki
AU - Takeuchi, Shoji
PY - 2012/1/1
Y1 - 2012/1/1
N2 - This paper describes a method to observe a single neuron in a three-dimensional (3D) neuronal tissue formed by bottom-up tissue construction. We first formed GFP-actin-transfected neurospheroids for visualization of neurons, and then formed a millimeter-sized neuronal block using PDMS mold chambers. We found that GFP-actin-transfected neurons in the neuronal block have in vivo-like morphology and 3D extension of their axons/dendrites compared to 2D culture system, and that many spines were successfully formed on the dendrites. We are confident that our neuronal block enables morphological maturation of the neurons due to its in vivo-like 3D environment, and that our bottom-up construction to observe single neurons is a powerful approach for various fields including analysis of single neuronal behaviors in vivo, and pharmacological assay for drug screening.
AB - This paper describes a method to observe a single neuron in a three-dimensional (3D) neuronal tissue formed by bottom-up tissue construction. We first formed GFP-actin-transfected neurospheroids for visualization of neurons, and then formed a millimeter-sized neuronal block using PDMS mold chambers. We found that GFP-actin-transfected neurons in the neuronal block have in vivo-like morphology and 3D extension of their axons/dendrites compared to 2D culture system, and that many spines were successfully formed on the dendrites. We are confident that our neuronal block enables morphological maturation of the neurons due to its in vivo-like 3D environment, and that our bottom-up construction to observe single neurons is a powerful approach for various fields including analysis of single neuronal behaviors in vivo, and pharmacological assay for drug screening.
KW - 3D tissue engineering
KW - Cortex
KW - GFP-Actin
KW - Neuron
KW - Transfection
UR - http://www.scopus.com/inward/record.url?scp=84901749587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901749587&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901749587
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1144
EP - 1146
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -