Small interfering RNA and gene silencing in transgenic mice and rats

Hidetoshi Hasuwa, Kazuhiro Kaseda, Thorbjorg Einarsdottir, Masaru Okabe

Research output: Contribution to journalArticlepeer-review

228 Citations (Scopus)


After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished.

Original languageEnglish
Pages (from-to)227-230
Number of pages4
JournalFEBS Letters
Issue number1-2
Publication statusPublished - 2002 Dec 4
Externally publishedYes


  • Gene silencing
  • RNA interference
  • RNA polymerase III promoter
  • Small interfering RNA
  • Transgenic animal

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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