TY - JOUR
T1 - Small molecules assisting eggshell calcium dissolution for embryonic bone formation
AU - Ito, Taku
AU - Kato, Suguru
AU - Kubo, Akiko
AU - Suematsu, Makoto
AU - Nakata, Masaya
AU - Saikawa, Yoko
N1 - Funding Information:
We thank Messrs. Akira Sasao and Sohei Yaguchi (Jonan Green System) for providing ostrich eggs. We also thank Mr. Yutaka Tahara (Oihama High School) for helpful advice regarding shell-less culture, Mr. Tomoaki Mitani (Keio University) for TEM observation, and Professors Hiroaki Imai and Yuya Oaki (Keio University) for technical advices. This work was partially supported by a Grant-in-Aid for Challenging Exploratory Research (25560406) from the Japan Society for the Promotion of Science (Y.S.) and Kieikai Research Foundation (Y.S.) 2017T018. Infrastructure for imaging mass spectroscopy was built up under the support of JST, ERATO Suematsu Gas Biology to which M.S. contributed until March 2015.
Funding Information:
We thank Messrs. Akira Sasao and Sohei Yaguchi (Jonan Green System) for providing ostrich eggs. We also thank Mr. Yutaka Tahara (Oihama High School) for helpful advice regarding shell-less culture, Mr. Tomoaki Mitani (Keio University) for TEM observation, and Professors Hiroaki Imai and Yuya Oaki (Keio University) for technical advices. This work was partially supported by a Grant-in-Aid for Challenging Exploratory Research ( 25560406 ) from the Japan Society for the Promotion of Science (Y.S.) and Kieikai Research Foundation (Y.S.) 2017T018 . Infrastructure for imaging mass spectroscopy was built up under the support of JST , ERATO Suematsu Gas Biology to which M.S. contributed until March 2015.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/1/31
Y1 - 2020/1/31
N2 - Calcium carbonate in avian eggshell is used as a calcium source for bone formation during embryonic development. We studied ostrich eggshells as a representative avian egg and found that the calcium reserve assembly (CRA) region, eventually dissolving for bone formation, consists of nano-granules comprising amorphous calcium carbonate (ACC) by careful microscopic observation. ACC is unstable phase of calcium carbonate with high solubility and is stabilized by additives. As such a stabilizer, we found small molecules from eggshell internal region. Particularly, the ACC was recreated by the formation of calcium carbonate in the presence of D-myo-inositol 4,5-bisphosphate (Ins(4,5)P2), isolated from ostrich eggshell for the first time in this study. Further mass spectrometric imaging technique revealed that Ins(4,5)P2 is localized at the CRA region, suggesting that this compound could play a major role that makes it easier to dissolve and internalize eggshell calcium during embryogenesis.
AB - Calcium carbonate in avian eggshell is used as a calcium source for bone formation during embryonic development. We studied ostrich eggshells as a representative avian egg and found that the calcium reserve assembly (CRA) region, eventually dissolving for bone formation, consists of nano-granules comprising amorphous calcium carbonate (ACC) by careful microscopic observation. ACC is unstable phase of calcium carbonate with high solubility and is stabilized by additives. As such a stabilizer, we found small molecules from eggshell internal region. Particularly, the ACC was recreated by the formation of calcium carbonate in the presence of D-myo-inositol 4,5-bisphosphate (Ins(4,5)P2), isolated from ostrich eggshell for the first time in this study. Further mass spectrometric imaging technique revealed that Ins(4,5)P2 is localized at the CRA region, suggesting that this compound could play a major role that makes it easier to dissolve and internalize eggshell calcium during embryogenesis.
KW - Amorphous calcium carbonate
KW - Eggshell
KW - Embryogenesis
KW - Inositol phosphate
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U2 - 10.1016/j.tet.2019.130854
DO - 10.1016/j.tet.2019.130854
M3 - Article
AN - SCOPUS:85076860057
SN - 0040-4020
VL - 76
JO - Tetrahedron
JF - Tetrahedron
IS - 5
M1 - 130854
ER -