Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells

Yoshimasa Saito; Gangning Liang; Gerda Egger; Jeffrey M. Friedman; Jody C. Chuang; Gerhard A. Coetzee; Peter A. Jones

doi:10.1016/j.ccr.2006.04.020

Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells

Yoshimasa Saito, Gangning Liang, Gerda Egger, Jeffrey M. Friedman, Jody C. Chuang, Gerhard A. Coetzee, Peter A. Jones

Research output: Contribution to journal › Article › peer-review

1194 Citations (Scopus)

Abstract

Expression profiling of T24 cells revealed that 17 out of 313 human miRNAs were upregulated more than 3-fold by simultaneous treatment with the chromatin-modifying drugs 5-aza-2′-deoxycytidine and 4-phenylbutyric acid. One of these, miR-127, is embedded in a CpG island and is highly induced from its own promoter after treatment. miR-127 is usually expressed as part of a miRNA cluster in normal cells but not in cancer cells, suggesting that it is subject to epigenetic silencing. In addition, the proto-oncogene BCL6, a potential target of miR-127, was translationally downregulated after treatment. These results suggest that DNA demethylation and histone deacetylase inhibition can activate expression of miRNAs that may act as tumor suppressors.

Original language	English
Pages (from-to)	435-443
Number of pages	9
Journal	Cancer Cell
Volume	9
Issue number	6
DOIs	https://doi.org/10.1016/j.ccr.2006.04.020
Publication status	Published - 2006 Jun 13
Externally published	Yes

Keywords

CELLCYCLE
DNA
RNA

ASJC Scopus subject areas

Oncology
Cell Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ccr.2006.04.020

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@article{2519f5f776bc43c4866eb8db32f8e968,

title = "Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells",

abstract = "Expression profiling of T24 cells revealed that 17 out of 313 human miRNAs were upregulated more than 3-fold by simultaneous treatment with the chromatin-modifying drugs 5-aza-2′-deoxycytidine and 4-phenylbutyric acid. One of these, miR-127, is embedded in a CpG island and is highly induced from its own promoter after treatment. miR-127 is usually expressed as part of a miRNA cluster in normal cells but not in cancer cells, suggesting that it is subject to epigenetic silencing. In addition, the proto-oncogene BCL6, a potential target of miR-127, was translationally downregulated after treatment. These results suggest that DNA demethylation and histone deacetylase inhibition can activate expression of miRNAs that may act as tumor suppressors.",

keywords = "CELLCYCLE, DNA, RNA",

author = "Yoshimasa Saito and Gangning Liang and Gerda Egger and Friedman, {Jeffrey M.} and Chuang, {Jody C.} and Coetzee, {Gerhard A.} and Jones, {Peter A.}",

note = "Funding Information: This work was supported by grants from the National Institutes of Health (RO1 CA 82422 to P.A.J.), the Department of Defense (W81XWH-04-1-0823 to G.A.C.), the Uehara Memorial Foundation of Japan (Y.S.), and the Max Kade Foundation (G.E.). We thank Yvonne C. Tsai and Tony W.H. Li for their assistance in isolating tissue RNA and DNA and critical reading of the manuscript, respectively. All tissue procurement was performed by the USC/Norris Comprehensive Cancer Center Translational Pathology Core Facility, supported by the Cancer Center Support Grant P30 CA 014089. ",

year = "2006",

month = jun,

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doi = "10.1016/j.ccr.2006.04.020",

language = "English",

volume = "9",

pages = "435--443",

journal = "Cancer Cell",

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T1 - Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells

AU - Saito, Yoshimasa

AU - Liang, Gangning

AU - Egger, Gerda

AU - Friedman, Jeffrey M.

AU - Chuang, Jody C.

AU - Coetzee, Gerhard A.

AU - Jones, Peter A.

N1 - Funding Information: This work was supported by grants from the National Institutes of Health (RO1 CA 82422 to P.A.J.), the Department of Defense (W81XWH-04-1-0823 to G.A.C.), the Uehara Memorial Foundation of Japan (Y.S.), and the Max Kade Foundation (G.E.). We thank Yvonne C. Tsai and Tony W.H. Li for their assistance in isolating tissue RNA and DNA and critical reading of the manuscript, respectively. All tissue procurement was performed by the USC/Norris Comprehensive Cancer Center Translational Pathology Core Facility, supported by the Cancer Center Support Grant P30 CA 014089.

PY - 2006/6/13

Y1 - 2006/6/13

N2 - Expression profiling of T24 cells revealed that 17 out of 313 human miRNAs were upregulated more than 3-fold by simultaneous treatment with the chromatin-modifying drugs 5-aza-2′-deoxycytidine and 4-phenylbutyric acid. One of these, miR-127, is embedded in a CpG island and is highly induced from its own promoter after treatment. miR-127 is usually expressed as part of a miRNA cluster in normal cells but not in cancer cells, suggesting that it is subject to epigenetic silencing. In addition, the proto-oncogene BCL6, a potential target of miR-127, was translationally downregulated after treatment. These results suggest that DNA demethylation and histone deacetylase inhibition can activate expression of miRNAs that may act as tumor suppressors.

AB - Expression profiling of T24 cells revealed that 17 out of 313 human miRNAs were upregulated more than 3-fold by simultaneous treatment with the chromatin-modifying drugs 5-aza-2′-deoxycytidine and 4-phenylbutyric acid. One of these, miR-127, is embedded in a CpG island and is highly induced from its own promoter after treatment. miR-127 is usually expressed as part of a miRNA cluster in normal cells but not in cancer cells, suggesting that it is subject to epigenetic silencing. In addition, the proto-oncogene BCL6, a potential target of miR-127, was translationally downregulated after treatment. These results suggest that DNA demethylation and histone deacetylase inhibition can activate expression of miRNAs that may act as tumor suppressors.

KW - CELLCYCLE

KW - DNA

KW - RNA

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Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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