Specificity of Presepsin as a Biomarker of Bacterial Infection in Mouse Sepsis Models

Kyosuke Hosokawa, Hideaki Obara, Kazumasa Fukuda, Kentaro Mastubara, Yuko Kitagawa

Research output: Contribution to journalArticlepeer-review


Introduction: Since its discovery in 2002, presepsin (P-SEP) has been reported to be useful in the early diagnosis of sepsis and has been evaluated in many clinical studies. However, as antibodies that bind to mouse P-SEP were previously unavailable, serum P-SEP levels in mice are limited. This study used a P-SEP enzyme-linked immunosorbent assay kit to evaluate the changes in serum P-SEP levels in mouse sepsis models compared with changes in other inflammatory markers and determine whether P-SEP can function as a biomarker specific to bacterial infections. Methods: Sepsis was induced in mice via cecal ligation and puncture (CLP), induction with lipopolysaccharide (LPS), and cecal ligation (CL) model was created as a control for the CLP model, following which clinical biomarkers (P-SEP, C-reactive protein, and procalcitonin) were evaluated. Results: The 48-h survival rates in the CLP, CL, and LPS-induced sepsis models were 67%, 89%, and 57%, respectively. Serum C-reactive protein levels did not increase in the CLP and CL models within 24 h but significantly increased in the LPS-induced sepsis model. Serum procalcitonin levels increased in the CLP and CL models and especially increased in the LPS-induced sepsis model. In contrast, an increase in serum P-SEP level was found in the CLP model at 6 h compared with those at baseline, the CL, and LPS-induced sepsis models. Conclusions: Mouse P-SEP is elevated early in infection and more specific to bacterial infection compared with other biomarkers.

Original languageEnglish
Pages (from-to)572-580
Number of pages9
JournalJournal of Surgical Research
Publication statusPublished - 2023 Mar


  • C-Reactive protein
  • Cecal ligation and puncture
  • Lipopolysaccharide
  • Presepsin
  • Procalcitonin
  • Sepsis

ASJC Scopus subject areas

  • Surgery


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