Split-and-contact device to form planar lipid bilayers

Y. Tsuji; R. Kawano; T. Osaki; K. Kamiya; N. Miki; S. Takeuchi

doi:10.1109/MEMSYS.2013.6474202

Split-and-contact device to form planar lipid bilayers

Y. Tsuji, R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

This paper describes a method to form bilayer lipid membranes by splitting, sliding and contacting water droplets in oil containing lipid. A droplet surrounded by a lipid monolayer, which is easily formed by injecting an aqueous droplet into an organic solvent that contains lipids, is split and then, brought into contact each other. A bilayer lipid membrane is immediately formed at the interface of the split droplets. These processes are conducted on a micro device which we termed as split-and-contact device. The aqueous droplet with a lipid monolayer is split mechanically by either spinning or sliding motions of the device. We experimentally verified the effectiveness of the proposed split-and-contact device by using α-hemolysin and its blockers (single-stranded DNA and hepta-6-sulfato β-cyclodextrin). This device can be readily applicable to highly efficient biological sensor or drug discovery.

Original language	English
Title of host publication	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages	161-164
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2013.6474202
Publication status	Published - 2013
Event	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China Duration: 2013 Jan 20 → 2013 Jan 24

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Country/Territory	Taiwan, Province of China
City	Taipei
Period	13/1/20 → 13/1/24

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2013.6474202

Cite this

Tsuji, Y., Kawano, R., Osaki, T., Kamiya, K., Miki, N., & Takeuchi, S. (2013). Split-and-contact device to form planar lipid bilayers. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 161-164). Article 6474202 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474202

Split-and-contact device to form planar lipid bilayers. / Tsuji, Y.; Kawano, R.; Osaki, T. et al.
IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 161-164 6474202 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tsuji, Y, Kawano, R, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2013, Split-and-contact device to form planar lipid bilayers. in IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013., 6474202, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 161-164, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 13/1/20. https://doi.org/10.1109/MEMSYS.2013.6474202

@inproceedings{10a2084fc59848c2bc40032cf75f0f36,

title = "Split-and-contact device to form planar lipid bilayers",

abstract = "This paper describes a method to form bilayer lipid membranes by splitting, sliding and contacting water droplets in oil containing lipid. A droplet surrounded by a lipid monolayer, which is easily formed by injecting an aqueous droplet into an organic solvent that contains lipids, is split and then, brought into contact each other. A bilayer lipid membrane is immediately formed at the interface of the split droplets. These processes are conducted on a micro device which we termed as split-and-contact device. The aqueous droplet with a lipid monolayer is split mechanically by either spinning or sliding motions of the device. We experimentally verified the effectiveness of the proposed split-and-contact device by using α-hemolysin and its blockers (single-stranded DNA and hepta-6-sulfato β-cyclodextrin). This device can be readily applicable to highly efficient biological sensor or drug discovery.",

author = "Y. Tsuji and R. Kawano and T. Osaki and K. Kamiya and N. Miki and S. Takeuchi",

note = "Copyright: Copyright 2013 Elsevier B.V., All rights reserved.; IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 ; Conference date: 20-01-2013 Through 24-01-2013",

year = "2013",

doi = "10.1109/MEMSYS.2013.6474202",

language = "English",

isbn = "9781467356558",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "161--164",

booktitle = "IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013",

}

TY - GEN

T1 - Split-and-contact device to form planar lipid bilayers

AU - Tsuji, Y.

AU - Kawano, R.

AU - Osaki, T.

AU - Kamiya, K.

AU - Miki, N.

AU - Takeuchi, S.

PY - 2013

Y1 - 2013

N2 - This paper describes a method to form bilayer lipid membranes by splitting, sliding and contacting water droplets in oil containing lipid. A droplet surrounded by a lipid monolayer, which is easily formed by injecting an aqueous droplet into an organic solvent that contains lipids, is split and then, brought into contact each other. A bilayer lipid membrane is immediately formed at the interface of the split droplets. These processes are conducted on a micro device which we termed as split-and-contact device. The aqueous droplet with a lipid monolayer is split mechanically by either spinning or sliding motions of the device. We experimentally verified the effectiveness of the proposed split-and-contact device by using α-hemolysin and its blockers (single-stranded DNA and hepta-6-sulfato β-cyclodextrin). This device can be readily applicable to highly efficient biological sensor or drug discovery.

AB - This paper describes a method to form bilayer lipid membranes by splitting, sliding and contacting water droplets in oil containing lipid. A droplet surrounded by a lipid monolayer, which is easily formed by injecting an aqueous droplet into an organic solvent that contains lipids, is split and then, brought into contact each other. A bilayer lipid membrane is immediately formed at the interface of the split droplets. These processes are conducted on a micro device which we termed as split-and-contact device. The aqueous droplet with a lipid monolayer is split mechanically by either spinning or sliding motions of the device. We experimentally verified the effectiveness of the proposed split-and-contact device by using α-hemolysin and its blockers (single-stranded DNA and hepta-6-sulfato β-cyclodextrin). This device can be readily applicable to highly efficient biological sensor or drug discovery.

UR - http://www.scopus.com/inward/record.url?scp=84875412063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875412063&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2013.6474202

DO - 10.1109/MEMSYS.2013.6474202

M3 - Conference contribution

AN - SCOPUS:84875412063

SN - 9781467356558

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 161

EP - 164

BT - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013

T2 - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013

Y2 - 20 January 2013 through 24 January 2013

ER -

Split-and-contact device to form planar lipid bilayers

Abstract

Publication series

Other

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this