Spontaneous apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells

Shinji Tsutsumi; Kazuhito Rokutan; Tomofusa Tsuchiya; Tohru Mizushima

doi:10.1023/A:1005404424698

Spontaneous apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells

Shinji Tsutsumi, Kazuhito Rokutan, Tomofusa Tsuchiya, Tohru Mizushima

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The purpose of this study was to elucidate the mechanism of spontaneous and rapid cell death of cultured gastric pit cells. Gastric pit cells have a rapid cell turnover rate in vivo. We here show that guinea pig gastric pit cells in culture undergo spontaneous and rapid apoptotic DNA fragmentation, which may represent the rapid cell turnover cycle of gastric pit cells in vivo. This spontaneous apoptotic DNA fragmentation required the presence of fetal calf serum in the culture media. Furthermore, the spontaneous apoptotic DNA fragmentation was prevented by protein synthesis and caspase inhibitors.

Original language	English
Pages (from-to)	291-297
Number of pages	7
Journal	Digestive Diseases and Sciences
Volume	45
Issue number	2
DOIs	https://doi.org/10.1023/A:1005404424698
Publication status	Published - 2000
Externally published	Yes

Keywords

Apoptosis
Caspase
Gastric mucosal cells
Pit cell lineage

ASJC Scopus subject areas

Physiology
Gastroenterology

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10.1023/A:1005404424698

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title = "Spontaneous apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells",

abstract = "The purpose of this study was to elucidate the mechanism of spontaneous and rapid cell death of cultured gastric pit cells. Gastric pit cells have a rapid cell turnover rate in vivo. We here show that guinea pig gastric pit cells in culture undergo spontaneous and rapid apoptotic DNA fragmentation, which may represent the rapid cell turnover cycle of gastric pit cells in vivo. This spontaneous apoptotic DNA fragmentation required the presence of fetal calf serum in the culture media. Furthermore, the spontaneous apoptotic DNA fragmentation was prevented by protein synthesis and caspase inhibitors.",

keywords = "Apoptosis, Caspase, Gastric mucosal cells, Pit cell lineage",

author = "Shinji Tsutsumi and Kazuhito Rokutan and Tomofusa Tsuchiya and Tohru Mizushima",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture, Japan and the Mochida Memorial Foundation for Medical and Pharmaceutical Research.",

year = "2000",

doi = "10.1023/A:1005404424698",

language = "English",

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pages = "291--297",

journal = "Digestive Diseases and Sciences",

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TY - JOUR

T1 - Spontaneous apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells

AU - Tsutsumi, Shinji

AU - Rokutan, Kazuhito

AU - Tsuchiya, Tomofusa

AU - Mizushima, Tohru

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture, Japan and the Mochida Memorial Foundation for Medical and Pharmaceutical Research.

PY - 2000

Y1 - 2000

N2 - The purpose of this study was to elucidate the mechanism of spontaneous and rapid cell death of cultured gastric pit cells. Gastric pit cells have a rapid cell turnover rate in vivo. We here show that guinea pig gastric pit cells in culture undergo spontaneous and rapid apoptotic DNA fragmentation, which may represent the rapid cell turnover cycle of gastric pit cells in vivo. This spontaneous apoptotic DNA fragmentation required the presence of fetal calf serum in the culture media. Furthermore, the spontaneous apoptotic DNA fragmentation was prevented by protein synthesis and caspase inhibitors.

AB - The purpose of this study was to elucidate the mechanism of spontaneous and rapid cell death of cultured gastric pit cells. Gastric pit cells have a rapid cell turnover rate in vivo. We here show that guinea pig gastric pit cells in culture undergo spontaneous and rapid apoptotic DNA fragmentation, which may represent the rapid cell turnover cycle of gastric pit cells in vivo. This spontaneous apoptotic DNA fragmentation required the presence of fetal calf serum in the culture media. Furthermore, the spontaneous apoptotic DNA fragmentation was prevented by protein synthesis and caspase inhibitors.

KW - Apoptosis

KW - Caspase

KW - Gastric mucosal cells

KW - Pit cell lineage

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DO - 10.1023/A:1005404424698

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AN - SCOPUS:0034053041

SN - 0163-2116

VL - 45

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EP - 297

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 2

ER -

Spontaneous apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells

Abstract

Keywords

ASJC Scopus subject areas

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