TY - JOUR
T1 - Stimulation of neurotrophic factor secretion from 1321N1 human astrocytoma cells by novel diterpenoids, scabronines A and G
AU - Obara, Yutaro
AU - Nakahata, Norimichi
AU - Kita, Takako
AU - Takaya, Yoshiaki
AU - Kobayashi, Hironori
AU - Hosoi, Shinzo
AU - Kiuchi, Fumiyuki
AU - Ohta, Tomihisa
AU - Oshima, Yoshiteru
AU - Ohizumi, Yasushi
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid from Scientific Research from the Ministry of Education, Science, Sport and Culture of Japan, Suzuken Memorial Foundation and the Research Foundation for Pharmaceutical Sciences.
PY - 1999/4/1
Y1 - 1999/4/1
N2 - Glial cells release neurotrophic factors that maintain neurons functionally. When rat pheochromocytoma cells (PC-12) were cultivated with the conditioned medium of human astrocytoma cells (1321N1) incubated with the new diterpenoids, scabronines A and G, isolated from Sarcodon scabrosus, they changed their morphology and there was neurite outgrowth. The scabronines increased the expression of mRNA for nerve growth factor (NGF), and the secretion of NGF from 1321N1 cells in a concentration-dependent manner. However, the enhanced neurite outgrowth produced by the conditioned media was slightly inhibited by NGF neutralizing antibody, and the concentration of NGF released in response to the scabronines was insufficient to cause differentiation. These results suggest that scabronines cause the secretion of other factors together with NGF from 1321N1 cells. The diterpenoids are useful drugs to clarify the mechanism of synthesis and secretion of neurotrophic factors. Copyright (C) 1999 Elsevier Science B.V.
AB - Glial cells release neurotrophic factors that maintain neurons functionally. When rat pheochromocytoma cells (PC-12) were cultivated with the conditioned medium of human astrocytoma cells (1321N1) incubated with the new diterpenoids, scabronines A and G, isolated from Sarcodon scabrosus, they changed their morphology and there was neurite outgrowth. The scabronines increased the expression of mRNA for nerve growth factor (NGF), and the secretion of NGF from 1321N1 cells in a concentration-dependent manner. However, the enhanced neurite outgrowth produced by the conditioned media was slightly inhibited by NGF neutralizing antibody, and the concentration of NGF released in response to the scabronines was insufficient to cause differentiation. These results suggest that scabronines cause the secretion of other factors together with NGF from 1321N1 cells. The diterpenoids are useful drugs to clarify the mechanism of synthesis and secretion of neurotrophic factors. Copyright (C) 1999 Elsevier Science B.V.
KW - 1321N1 human astrocytoma cell
KW - NGF (nerve growth factor)
KW - Neurite outgrowth
KW - Neurotrophic factor
KW - PC-12 cell
KW - Scabronine
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U2 - 10.1016/S0014-2999(99)00077-1
DO - 10.1016/S0014-2999(99)00077-1
M3 - Article
C2 - 10323283
AN - SCOPUS:0032898427
SN - 0014-2999
VL - 370
SP - 79
EP - 84
JO - European journal of pharmacology
JF - European journal of pharmacology
IS - 1
ER -