Abstract
A glycosphingolipid analogue (12-azidododecyl β-lactoside) as a saccharide primer has been shown to be useful for the synthesis of oligosaccharide libraries by mammalian cells. In the present study, CE-ESI-MS was employed to elucidate the structure of glycosphingolipid analogues derived from 12-azidododecyl b-lactoside (Lac-C12N3) by mammalian cells. MDCK cells and COLO201 cells were cultured with Lac-C12N3, and the glycosylated products secreted into the medium were collected and separated into acidic and neutral products by column chromatography. The acidic products could be directly analyzed by CEESI-MS, while the neutral products were converted to anionic derivatives via a reaction with propiolic acid. With this method, it was possible to analyze both acidic and neutral products glycosylated by MDCK cells and COLO201 cells at high sensitivity.
| Original language | English |
|---|---|
| Pages (from-to) | 3519-3526 |
| Number of pages | 8 |
| Journal | ELECTROPHORESIS |
| Volume | 30 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 2009 |
Keywords
- CE-ESI-MS
- Neutral carbohydrate
- Saccharide primer
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
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