Subcultured astrocytes suppress the proliferation of neuroblasts in vitro

Kazunari Yoshida, Shinichi Kohsaka, Seiji Nii, Takeshi Idei, Mitsuhiro Otani, Shigeo Toya, Yasuzo Tsukada

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


The effects of subcultured astrocytes on the proliferation of neuronal precursor cells (neuroblasts) from rat embryonic cerebral hemispheres were examined. The survivability of neurons and the neurite outgrowth were significantly enhanced by the subcultured astrocytes compared to those of neurons plated on poly-l-lysine-coated coverslips. The incorporation of [3H]thymidine into neuroblasts was remarkably suppressed by the subcultured astrocytes indicating that the astrocytes inhibit the proliferation of neuroblasts. These results suggest that astrocytes enhance the maturation of neuroblasts possibly via either cell-cell contact or trophic substances.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalNeuroscience Letters
Issue number1
Publication statusPublished - 1986 Sept 25
Externally publishedYes


  • Neuroblast
  • Neuron-glia interaction
  • Neurotrophic factor
  • Proliferation
  • Tissue culture

ASJC Scopus subject areas

  • Neuroscience(all)


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