Subcultured astrocytes suppress the proliferation of neuroblasts in vitro

Kazunari Yoshida; Shinichi Kohsaka; Seiji Nii; Takeshi Idei; Mitsuhiro Otani; Shigeo Toya; Yasuzo Tsukada

doi:10.1016/0304-3940(86)90433-7

Subcultured astrocytes suppress the proliferation of neuroblasts in vitro

Kazunari Yoshida, Shinichi Kohsaka, Seiji Nii, Takeshi Idei, Mitsuhiro Otani, Shigeo Toya, Yasuzo Tsukada

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The effects of subcultured astrocytes on the proliferation of neuronal precursor cells (neuroblasts) from rat embryonic cerebral hemispheres were examined. The survivability of neurons and the neurite outgrowth were significantly enhanced by the subcultured astrocytes compared to those of neurons plated on poly-l-lysine-coated coverslips. The incorporation of [³H]thymidine into neuroblasts was remarkably suppressed by the subcultured astrocytes indicating that the astrocytes inhibit the proliferation of neuroblasts. These results suggest that astrocytes enhance the maturation of neuroblasts possibly via either cell-cell contact or trophic substances.

Original language	English
Pages (from-to)	34-39
Number of pages	6
Journal	Neuroscience Letters
Volume	70
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(86)90433-7
Publication status	Published - 1986 Sept 25
Externally published	Yes

Keywords

Neuroblast
Neuron-glia interaction
Neurotrophic factor
Proliferation
Tissue culture

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0304-3940(86)90433-7

Cite this

@article{4fc5d6056ea94fa98746fcebefa506f3,

title = "Subcultured astrocytes suppress the proliferation of neuroblasts in vitro",

abstract = "The effects of subcultured astrocytes on the proliferation of neuronal precursor cells (neuroblasts) from rat embryonic cerebral hemispheres were examined. The survivability of neurons and the neurite outgrowth were significantly enhanced by the subcultured astrocytes compared to those of neurons plated on poly-l-lysine-coated coverslips. The incorporation of [3H]thymidine into neuroblasts was remarkably suppressed by the subcultured astrocytes indicating that the astrocytes inhibit the proliferation of neuroblasts. These results suggest that astrocytes enhance the maturation of neuroblasts possibly via either cell-cell contact or trophic substances.",

keywords = "Neuroblast, Neuron-glia interaction, Neurotrophic factor, Proliferation, Tissue culture",

author = "Kazunari Yoshida and Shinichi Kohsaka and Seiji Nii and Takeshi Idei and Mitsuhiro Otani and Shigeo Toya and Yasuzo Tsukada",

note = "Funding Information: ent study was partially supported by grants from the Ministry of Education, Science and Culture, the Japan Brain Foundation, and from the Takeda Science Foundation.",

year = "1986",

month = sep,

day = "25",

doi = "10.1016/0304-3940(86)90433-7",

language = "English",

volume = "70",

pages = "34--39",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Subcultured astrocytes suppress the proliferation of neuroblasts in vitro

AU - Yoshida, Kazunari

AU - Kohsaka, Shinichi

AU - Nii, Seiji

AU - Idei, Takeshi

AU - Otani, Mitsuhiro

AU - Toya, Shigeo

AU - Tsukada, Yasuzo

N1 - Funding Information: ent study was partially supported by grants from the Ministry of Education, Science and Culture, the Japan Brain Foundation, and from the Takeda Science Foundation.

PY - 1986/9/25

Y1 - 1986/9/25

N2 - The effects of subcultured astrocytes on the proliferation of neuronal precursor cells (neuroblasts) from rat embryonic cerebral hemispheres were examined. The survivability of neurons and the neurite outgrowth were significantly enhanced by the subcultured astrocytes compared to those of neurons plated on poly-l-lysine-coated coverslips. The incorporation of [3H]thymidine into neuroblasts was remarkably suppressed by the subcultured astrocytes indicating that the astrocytes inhibit the proliferation of neuroblasts. These results suggest that astrocytes enhance the maturation of neuroblasts possibly via either cell-cell contact or trophic substances.

AB - The effects of subcultured astrocytes on the proliferation of neuronal precursor cells (neuroblasts) from rat embryonic cerebral hemispheres were examined. The survivability of neurons and the neurite outgrowth were significantly enhanced by the subcultured astrocytes compared to those of neurons plated on poly-l-lysine-coated coverslips. The incorporation of [3H]thymidine into neuroblasts was remarkably suppressed by the subcultured astrocytes indicating that the astrocytes inhibit the proliferation of neuroblasts. These results suggest that astrocytes enhance the maturation of neuroblasts possibly via either cell-cell contact or trophic substances.

KW - Neuroblast

KW - Neuron-glia interaction

KW - Neurotrophic factor

KW - Proliferation

KW - Tissue culture

UR - http://www.scopus.com/inward/record.url?scp=0022495903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022495903&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(86)90433-7

DO - 10.1016/0304-3940(86)90433-7

M3 - Article

C2 - 3774217

AN - SCOPUS:0022495903

SN - 0304-3940

VL - 70

SP - 34

EP - 39

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1

ER -

Subcultured astrocytes suppress the proliferation of neuroblasts in vitro

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this