Sulfation of estradiol in human epidermal keratinocyte

Akira Kushida, Kenji Hattori, Nozomi Yamaguchi, Tetsuyuki Kobayashi, Akira Date, Hiroomi Tamura

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Epidermis is one of the well-known estrogen target tissues. Information regarding estrogen metabolism in epidermis is still very limited compared to that of estrogen action. In the breast cancer tissue, 17β-estradiol (E 2) is inactivated by sulfation and the expression level of estrogen sulfotransferase (SULT1E1) is inversely correlated with its malignancy. However, there is little datum about inactivation of estradiol in skin. In order to detect and measure E 2 and its metabolites simultaneously, we established an assay method with radio HPLC. A majority of [ 3H] labeled E 2 was converted to E 2 sulfate in normal human epidermal keratinocyte (NHEK) cells. The estimated activity of sulfotransferase toward E 2 at 20 nM was 0.11±0.01 (pmol/min/mg protein). Significant induction of estrogen sulfotransferase activity was observed in calcium-differentiated NHEK cells (0.58±0.07 (pmol/min/mg protein)). The gene expression of SULT1E1 was fifteen-fold higher in differentiated keratinocyte than in proliferating keratinocyte, whereas that of steroid sulfatase was reduced. These results suggest that E 2 inactivation is primarily mediated by SULT1E1 in keratinocyte and E 2 action is likely suppressed in epidermal differentiation.

Original languageEnglish
Pages (from-to)1147-1151
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Issue number7
Publication statusPublished - 2011 Jul


  • Estrogen
  • Keratinocyte
  • Sulfotransferase

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science


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