31P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress

Yuichi Takeda; Tomoko Ogawa; Yoshiyuki Nakamura; Kunihiro Kasamo; Makoto Sakata; Eiji Ohta

doi:10.1093/oxfordjournals.pcp.a078832

³¹P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress

Yuichi Takeda, Tomoko Ogawa, Yoshiyuki Nakamura, Kunihiro Kasamo, Makoto Sakata, Eiji Ohta

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Intracellular pH and levels of ATP in intact root-tip cells of mung bean (Vigna mungo [L.] Hepper) under low-temperature stress were investigated in vivo by ³¹P nuclear magnetic resonance (³¹P-NMR) spectroscopy. Root-tips of 3 mm in length were excised from seedlings of mung bean that had been chilled at 0°C after grown at 30°C. Chilling for longer than 12 h caused changes in the intracellular pH and decreased levels of ATP in the seedlings. The level of ATP recovered within 30 min but little change in pH was observed when samples were rewarmed to 20° C after chilling at 5°C. However, after chilling for longer than 48 h, neither the intracellular pH nor the level of ATP was restored.These results suggest that a decline in the activity of tonoplast H⁺-ATPase, induced by chillings, might be a significant early event in cold-induced injury that leads to cell damage.

Original language	English
Pages (from-to)	865-871
Number of pages	7
Journal	Plant and Cell Physiology
Volume	36
Issue number	5
DOIs	https://doi.org/10.1093/oxfordjournals.pcp.a078832
Publication status	Published - 1995 Jul
Externally published	Yes

Keywords

31P nuclear magnetic resonance
Cold-induced injury
Intracellular pH
Level of ATP
Low-temperature stress
Mung bean {Vigna mungo)

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

Access to Document

10.1093/oxfordjournals.pcp.a078832

Cite this

@article{4a417a74dd58471289c01cc7af9fd124,

title = "31P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress",

abstract = "Intracellular pH and levels of ATP in intact root-tip cells of mung bean (Vigna mungo [L.] Hepper) under low-temperature stress were investigated in vivo by 31P nuclear magnetic resonance (31P-NMR) spectroscopy. Root-tips of 3 mm in length were excised from seedlings of mung bean that had been chilled at 0°C after grown at 30°C. Chilling for longer than 12 h caused changes in the intracellular pH and decreased levels of ATP in the seedlings. The level of ATP recovered within 30 min but little change in pH was observed when samples were rewarmed to 20° C after chilling at 5°C. However, after chilling for longer than 48 h, neither the intracellular pH nor the level of ATP was restored.These results suggest that a decline in the activity of tonoplast H+-ATPase, induced by chillings, might be a significant early event in cold-induced injury that leads to cell damage.",

keywords = "31P nuclear magnetic resonance, Cold-induced injury, Intracellular pH, Level of ATP, Low-temperature stress, Mung bean {Vigna mungo)",

author = "Yuichi Takeda and Tomoko Ogawa and Yoshiyuki Nakamura and Kunihiro Kasamo and Makoto Sakata and Eiji Ohta",

note = "Funding Information: The authors are grateful to Prof. S. Yoshida of Low Temperature Science, Hokkaido University, Sapporo, Japan, for helpful advice related to this study and to Prof. T. Sato of Keio University for his encouragement. This work was supported in part by a Orant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.",

year = "1995",

month = jul,

doi = "10.1093/oxfordjournals.pcp.a078832",

language = "English",

volume = "36",

pages = "865--871",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

publisher = "Oxford University Press",

number = "5",

}

TY - JOUR

T1 - 31P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress

AU - Takeda, Yuichi

AU - Ogawa, Tomoko

AU - Nakamura, Yoshiyuki

AU - Kasamo, Kunihiro

AU - Sakata, Makoto

AU - Ohta, Eiji

N1 - Funding Information: The authors are grateful to Prof. S. Yoshida of Low Temperature Science, Hokkaido University, Sapporo, Japan, for helpful advice related to this study and to Prof. T. Sato of Keio University for his encouragement. This work was supported in part by a Orant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.

PY - 1995/7

Y1 - 1995/7

N2 - Intracellular pH and levels of ATP in intact root-tip cells of mung bean (Vigna mungo [L.] Hepper) under low-temperature stress were investigated in vivo by 31P nuclear magnetic resonance (31P-NMR) spectroscopy. Root-tips of 3 mm in length were excised from seedlings of mung bean that had been chilled at 0°C after grown at 30°C. Chilling for longer than 12 h caused changes in the intracellular pH and decreased levels of ATP in the seedlings. The level of ATP recovered within 30 min but little change in pH was observed when samples were rewarmed to 20° C after chilling at 5°C. However, after chilling for longer than 48 h, neither the intracellular pH nor the level of ATP was restored.These results suggest that a decline in the activity of tonoplast H+-ATPase, induced by chillings, might be a significant early event in cold-induced injury that leads to cell damage.

AB - Intracellular pH and levels of ATP in intact root-tip cells of mung bean (Vigna mungo [L.] Hepper) under low-temperature stress were investigated in vivo by 31P nuclear magnetic resonance (31P-NMR) spectroscopy. Root-tips of 3 mm in length were excised from seedlings of mung bean that had been chilled at 0°C after grown at 30°C. Chilling for longer than 12 h caused changes in the intracellular pH and decreased levels of ATP in the seedlings. The level of ATP recovered within 30 min but little change in pH was observed when samples were rewarmed to 20° C after chilling at 5°C. However, after chilling for longer than 48 h, neither the intracellular pH nor the level of ATP was restored.These results suggest that a decline in the activity of tonoplast H+-ATPase, induced by chillings, might be a significant early event in cold-induced injury that leads to cell damage.

KW - 31P nuclear magnetic resonance

KW - Cold-induced injury

KW - Intracellular pH

KW - Level of ATP

KW - Low-temperature stress

KW - Mung bean {Vigna mungo)

UR - http://www.scopus.com/inward/record.url?scp=0028891670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028891670&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.pcp.a078832

DO - 10.1093/oxfordjournals.pcp.a078832

M3 - Article

AN - SCOPUS:0028891670

SN - 0032-0781

VL - 36

SP - 865

EP - 871

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 5

ER -