Suppression of non-random fertilization by mhc class i antigens

Junki Kamiya, Woojin Kang, Keiichi Yoshida, Ryota Takagi, Seiya Kanai, Maito Hanai, Akihiro Nakamura, Mitsutoshi Yamada, Yoshitaka Miyamoto, Mami Miyado, Yoko Kuroki, Yoshiki Hayashi, Akihiro Umezawa, Natsuko Kawano, Kenji Miyado

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Hermaphroditic invertebrates and plants have a self-recognition system on the cell surface of sperm and eggs, which prevents their self-fusion and enhances non-self-fusion, thereby contributing to genetic variation. However, the system of sperm–egg recognition in mammals is under debate. To address this issue, we explored the role of major histocompatibility complex class I (MHC class I, also known as histocompatibility 2-Kb or H2-Kb and H2-Db in mice) antigens by analyzing H2-Kb-/- H2-Db-/- β2-microglobulin (β2M)-/- triple-knockout (T-KO) male mice with full fertility. T-KO sperm exhibited an increased sperm number in the perivitelline space of wild-type (WT) eggs in vitro. Moreover, T-KO sperm showed multiple fusion with zona pellucida (ZP)-free WT eggs, implying that the ability of polyspermy block for sperm from T-KO males was weakened in WT eggs. When T-KO male mice were intercrossed with WT female mice, the percentage of females in progeny increased. We speculate that WT eggs prefer fusion with T-KO sperm, more specifically X-chromosome-bearing sperm (X sperm), suggesting the presence of preferential (non-random) fertilization in mammals, including humans.

Original languageEnglish
Article number8731
Pages (from-to)1-13
Number of pages13
JournalInternational journal of molecular sciences
Issue number22
Publication statusPublished - 2020 Nov 2


  • MHC class I
  • Non-random fertilization
  • Polyspermy block
  • Sex ratio

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


Dive into the research topics of 'Suppression of non-random fertilization by mhc class i antigens'. Together they form a unique fingerprint.

Cite this