Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

Kanaya Eiko; Higashizaki Takako; Ozawa Fumiko; Hirai Keiko; Nishizawa Masafumi; Tokiinaga Masao; Tsukui Hiroko; Hatanaka Hiroshi; Hishinuma Fumio

doi:10.1016/0378-1119(89)90404-6

Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

Kanaya Eiko, Higashizaki Takako, Ozawa Fumiko, Hirai Keiko, Nishizawa Masafumi, Tokiinaga Masao, Tsukui Hiroko, Hatanaka Hiroshi, Hishinuma Fumio

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.

Original language	English
Pages (from-to)	65-74
Number of pages	10
Journal	Gene
Volume	83
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(89)90404-6
Publication status	Published - 1989 Nov 15
Externally published	Yes

Keywords

(Recombinant DNA
expression in yeast
neurite outgrowth)
pheochromocytoma cell
pre-pro sequence
processing of precursor protein

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(89)90404-6

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abstract = "The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.",

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T1 - Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

AU - Eiko, Kanaya

AU - Takako, Higashizaki

AU - Fumiko, Ozawa

AU - Keiko, Hirai

AU - Masafumi, Nishizawa

AU - Masao, Tokiinaga

AU - Hiroko, Tsukui

AU - Hiroshi, Hatanaka

AU - Fumio, Hishinuma

PY - 1989/11/15

Y1 - 1989/11/15

N2 - The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.

AB - The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast α-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.

KW - (Recombinant DNA

KW - expression in yeast

KW - neurite outgrowth)

KW - pheochromocytoma cell

KW - pre-pro sequence

KW - processing of precursor protein

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Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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