Synthetic Biomarker Design by Using Analyte-Responsive Acetaminophen

Tatsuya Nishihara; Joe Inoue; Sho Tabata; Shinnosuke Murakami; Takamasa Ishikawa; Natsumi Saito; Shinji Fukuda; Masaru Tomita; Tomoyoshi Soga

doi:10.1002/cbic.201700023

Synthetic Biomarker Design by Using Analyte-Responsive Acetaminophen

Tatsuya Nishihara, Joe Inoue, Sho Tabata, Shinnosuke Murakami, Takamasa Ishikawa, Natsumi Saito, Shinji Fukuda, Masaru Tomita, Tomoyoshi Soga

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte-responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof-of-concept, we designed hydrogen peroxide (H₂O₂)-responsive APAP (HR-APAP) and succeeded in H₂O₂ detection with cellular and animal experiments. In fact, for blood samples following HR-APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR-APAP] accurately reflects in vivo differences in H₂O₂ levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers.

Original language	English
Pages (from-to)	910-913
Number of pages	4
Journal	ChemBioChem
Volume	18
Issue number	10
DOIs	https://doi.org/10.1002/cbic.201700023
Publication status	Published - 2017 May 18

Keywords

acetaminophen
hydrogen peroxide
liquid chromatography
mass spectrometry
synthetic biomarker

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.201700023

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title = "Synthetic Biomarker Design by Using Analyte-Responsive Acetaminophen",

abstract = "The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte-responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof-of-concept, we designed hydrogen peroxide (H2O2)-responsive APAP (HR-APAP) and succeeded in H2O2 detection with cellular and animal experiments. In fact, for blood samples following HR-APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR-APAP] accurately reflects in vivo differences in H2O2 levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers.",

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author = "Tatsuya Nishihara and Joe Inoue and Sho Tabata and Shinnosuke Murakami and Takamasa Ishikawa and Natsumi Saito and Shinji Fukuda and Masaru Tomita and Tomoyoshi Soga",

note = "Funding Information: This work was supported by the AMED-CREST from the Japan Agency for Medical Research and Development, AMED (T.S.), Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP15J03449 (T.N.), and research funds from the Yamagata prefectural government and the City of Tsuruoka. T.N. was funded by a fellowship from the JSPS. We also thank Takahiro Masuda of Keio University for help with the animal experiments. Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

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AU - Inoue, Joe

AU - Tabata, Sho

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AU - Ishikawa, Takamasa

AU - Saito, Natsumi

AU - Fukuda, Shinji

AU - Tomita, Masaru

AU - Soga, Tomoyoshi

N1 - Funding Information: This work was supported by the AMED-CREST from the Japan Agency for Medical Research and Development, AMED (T.S.), Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP15J03449 (T.N.), and research funds from the Yamagata prefectural government and the City of Tsuruoka. T.N. was funded by a fellowship from the JSPS. We also thank Takahiro Masuda of Keio University for help with the animal experiments. Publisher Copyright: © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

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Synthetic Biomarker Design by Using Analyte-Responsive Acetaminophen

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Keywords

ASJC Scopus subject areas

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