@article{5c90b33ca9c647b3879a407aef857790,
title = "TAKASAGO-6 apparatus for cryogenic coherent X-ray diffraction imaging of biological non-crystalline particles using X-ray free electron laser at SACLA",
abstract = "Coherent X-ray diffraction imaging (CXDI) is a technique for structure analyses of non-crystalline particles with dimensions ranging from micrometer to sub-micrometer. We have developed a diffraction apparatus named TAKASAGO-6 for use in single-shot CXDI experiments of frozen-hydrated non-crystalline biological particles at cryogenic temperature with X-ray free electron laser pulses provided at a repetition rate of 30 Hz from the SPring-8 Angstrom Compact free-electron LAser. Specimen particles are flash-cooled after being dispersed on thin membranes supported by specially designed disks. The apparatus is equipped with a high-speed translation stage with a cryogenic pot for raster-scanning of the disks at a speed higher than 25 μm/33 ms. In addition, we use devices assisting the easy transfer of cooled specimens from liquid-nitrogen storages to the cryogenic pot. In the current experimental procedure, more than 20 000 diffraction patterns can be collected within 1 h. Here we report the key components and performance of the diffraction apparatus. Based on the efficiency of the diffraction data collection and the structure analyses of metal particles, biological cells, and cellular organelles, we discuss the future application of this diffraction apparatus for structure analyses of biological specimens.",
author = "Amane Kobayashi and Yuki Sekiguchi and Yuki Takayama and Tomotaka Oroguchi and Keiya Shirahama and Yasufumi Torizuka and Masahiro Manoda and Masayoshi Nakasako and Masaki Yamamoto",
note = "Funding Information: The construction and development of the TAKASAGO-6 apparatus was supported by the grant for XFEL key technology to M.N. and M.Y. and by the X-ray Free Electron Laser Priority Strategy Program from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) to M.N. and M.Y. In addition, specimen preparation and software development are supported by the grants from the Japan Society for the Promotion of Science to M.N. (Grant Nos. 1920402, 22018027, and 2465414), to Y.S. (Grant No. 15J01707), to A.K. (Grant No. 15J01831), and to Y.T. (Grant No. 25891033) and from the MEXT to M.N. (Grant Nos. 15076210, 20050030, and 22244054) and to T.O. (Grant No. 24113723). Y.T. was employed by the RIKEN Special Postdoctoral Researchers Program. The XFEL-CXDI diffraction data for structure analyses were collected at SACLA (Proposal Nos. 2014A8033, 2014B8052, and 2015A8051). The phase-retrieval calculations and multivariate analyses were performed using the mini-K supercomputer system at the SACLA facility. Publisher Copyright: {\textcopyright} 2016 Author(s).",
year = "2016",
month = may,
day = "1",
doi = "10.1063/1.4948317",
language = "English",
volume = "87",
journal = "Review of Scientific Instruments",
issn = "0034-6748",
publisher = "American Institute of Physics Publising LLC",
number = "5",
}