TY - JOUR
T1 - TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD
AU - Saito, Kuniaki
AU - Fujiwara, Toshinobu
AU - Katahira, Jun
AU - Inoue, Kunio
AU - Sakamoto, Hiroshi
N1 - Funding Information:
We thank Dr. Hans J. Gross for critical comments and advice on the manuscript. This work was supported by the research Grant from MEXT (No. 14035235) to H.S.
PY - 2004/8/20
Y1 - 2004/8/20
N2 - Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3′-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells.
AB - Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3′-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells.
KW - AU-rich element
KW - HuD
KW - Neuron
KW - TAP
KW - mRNA export
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U2 - 10.1016/j.bbrc.2004.06.140
DO - 10.1016/j.bbrc.2004.06.140
M3 - Article
C2 - 15358174
AN - SCOPUS:3242808974
SN - 0006-291X
VL - 321
SP - 291
EP - 297
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -