@article{ef9c0106662c4edc873e83738ea52548,
title = "Target protein-oriented isolation of Hes1 dimer inhibitors using protein based methods",
abstract = "Natural products isolation using protein based methods is an attractive for obtaining bioactive compounds. To discover neural stem cell (NSC) differentiation activators, we isolated eight inhibitors of Hes1 dimer formation from Psidium guajava using the Hes1-Hes1 interaction fluorescent plate assay and one inhibitor from Terminalia chebula using the Hes1-immobilized beads method. Of the isolated compounds, gallic acid (8) and 4-O-(4”-O-galloyl-α-L-rhamnopyranosyl)ellagic acid (11) showed potent Hes1 dimer formation inhibitory activity, with IC50 values of 10.3 and 2.53 μM, respectively. Compound 11 accelerated the differentiation activity of C17.2 NSC cells dose dependently, increasing the number of neurons with a 125% increase (5 μM) compared to the control.",
author = "Arai, {Midori A.} and Kaori Morita and Haruka Kawano and Yuna Makita and Manami Hashimoto and Akiko Suganami and Yutaka Tamura and Sadhu, {Samir K.} and Firoj Ahmed and Masami Ishibashi",
note = "Funding Information: This study was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS), Strategic Priority Research Promotion Program, Chiba University, “Phytochemical Plant Molecular Sciences”, Takeda Science Foundation, the Naito Foundation, Astellas Foundation for Research on Metabolic Disorders, the Uehara Memorial Foundation, and a Workshop on Chirality at Chiba University (WCCU). This work was inspired by the international and interdisciplinary environments of the JSPS Core-to-Core Program “Asian Chemical Biology Initiative” and the JSPS A3 Foresight Program. Publisher Copyright: {\textcopyright} 2020, The Author(s).",
year = "2020",
month = dec,
day = "1",
doi = "10.1038/s41598-020-58451-3",
language = "English",
volume = "10",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}