TY - JOUR
T1 - Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves
AU - Satoh, Yasushi
AU - Kanda, Yasunari
AU - Terakawa, Mitsuhiro
AU - Obara, Minoru
AU - Mizuno, Katsushige
AU - Watanabe, Yasuhiro
AU - Endo, Shogo
AU - Ooigawa, Hidetoshi
AU - Nawashiro, Hiroshi
AU - Sato, Shunichi
AU - Takishima, Kunio
N1 - Funding Information:
This work was partly supported by grants from the Japanese Ministry of Education, Science and Technology.
PY - 2005/11
Y1 - 2005/11
N2 - We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP reside at least 3.5 mm from the surface of the tissue, while no apparent damage was detected. Additionally, expression of EGFP was limited to the area that was exposed to LISWs. Using this method, the formulation of plasmid DNA by cationic transfer reagent polyethylen-imine proved to be effective for improving transfer efficiency into the CNS.
AB - We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP reside at least 3.5 mm from the surface of the tissue, while no apparent damage was detected. Additionally, expression of EGFP was limited to the area that was exposed to LISWs. Using this method, the formulation of plasmid DNA by cationic transfer reagent polyethylen-imine proved to be effective for improving transfer efficiency into the CNS.
KW - Central nervous system
KW - In vivo transfection
KW - Laser-induced stress wave
KW - Polyethylenimine
KW - Stereo-taxic injection
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U2 - 10.1117/1.2128432
DO - 10.1117/1.2128432
M3 - Article
C2 - 16409064
AN - SCOPUS:33645239667
SN - 1083-3668
VL - 10
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 6
M1 - 060501
ER -