Temperature-Responsive Chromatography Using Poly-(N-isopropylacrylamide) Hydrogel-Modified Silica

Hideko Kanazawa, Tatsuo Sunamoto, Eri Ayano, Yoshikazu Matsushima, Akihiko Kikuchi, Teruo Okano

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)


Poly(N-isopropylacrylamide) (PNIPAAm) has the sharpest phase transition of the class of thermo-sensitive N-alkyl acrylamide polymers. We developed a new method of HPLC using packing materials modified with cross-linked poly(N-isopropylacrylamide) (PNIPAAm) hydrogel. A temperature-responsive surface was prepared by polymerization of NIPAAm in the presence of a cross-linker on the silica support. The surface properties and functions of the stationary phases change in response to the external temperature. Therefore it easily changes the interaction of a solute with the surface with a constant aqueous mobile phase. A temperature-responsive elution behavior was observed on the separation of steroids and PTH-amino acids. The method is expected to be applicable to separation in the pharmaceutical and biomedical fields.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
Journalanalytical sciences
Issue number1
Publication statusPublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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