Temperature-responsive smart packing materials utilizing multi-functional polymers

Eri Ayano, Hideko Kanazawa

Research output: Contribution to journalReview articlepeer-review

18 Citations (Scopus)


Polymers that respond to small changes in environmental stimuli with large, sometimes discontinuous changes in their physical state or properties, are often called "smart" polymers. Poly(N-isopropylacrylamide), PNIPAAm, is one of the most representative smart polymer that exhibits a thermally reversible soluble-insoluble change in the vicinity of its lower critical solution temperature (LCST) at 32°C in aqueous solution. Temperature-responsive chromatography for the separation of biomolecules utilizing the poly(N-isopropylacrylamide) (PNIPAAm)-modified stationary phase is performed with an aqueous mobile phase without using an organic solvent. The surface properties and function of the stationary phase are controlled by external temperature changes without changing the mobile-phase composition. The separation of the biomolecules, such as nucleotides, was achieved by a dual temperature- and pH-responsive chromatography system. The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase. Additionally, we also prepared functional copolymers composed of N-isopropylacrylamide (NIPAAm) and amino acid derivative or naphthyl alanine derivative, which have temperature-responsiveness and molecular recognition. These separation systems would have potential applications in the separation of biomolecules.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
Journalanalytical sciences
Issue number1
Publication statusPublished - 2014 Jan
Externally publishedYes


  • A dual temperature- and pH-responsive chromatography
  • Bio-separation
  • Functional polymer
  • Molecular recognition
  • Poly(N-isopropylacrylamide)
  • Temperature-responsive chromatography

ASJC Scopus subject areas

  • Analytical Chemistry


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