Temperature-responsive solid-phase extraction column for biological sample pretreatment

Michiko Akimaru, Kohei Okubo, Yuki Hiruta, Hideko Kanazawa

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


We have developed a novel solid-phase extraction (SPE) system utilizing a temperature-responsive polymer hydrogelmodified stationary phase. Aminopropyl silica beads (average diameter, 40 - 64 μm) were coated with poly(N-isopropylacrylamide) (PNIPAAm)-based thermo-responsive hydrogels. Butyl methacrylate (BMA) and N,N-dimethylaminopropyl acrylamide (DMAPAAm) were used as the hydrophobic and cationic monomers, respectively, and copolymerized with NIPAAm. To evaluate the use of this SPE cartridge for the analysis of drugs and proteins in biological fluids, we studied the separation of phenytoin and theophylline from human serum albumin (HSA) as a model system. The retention of the analytes in an exclusively aqueous eluent could be modulated by changing the temperature and salt content. These results indicated that this temperature-responsive SPE system can be applied to the pretreatment of biological samples for the measurement of serum drug levels.

Original languageEnglish
Pages (from-to)881-886
Number of pages6
Journalanalytical sciences
Issue number9
Publication statusPublished - 2015


  • Human serum albumin
  • Phenytoin
  • Poly(N-isopropylacrylamide)
  • Pretreatment
  • Protein
  • Solid phase extraction
  • Temperature-responsive packing materials
  • Theophylline

ASJC Scopus subject areas

  • Analytical Chemistry


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