The BAH domain facilitates the ability of human Orc1 protein to activate replication origins in vivo

Kohji Noguchi, Alex Vassilev, Soma Ghosh, John L. Yates, Melvin L. DePamphilis

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)


Selection of initiation sites for DNA replication in eukaryotes is determined by the interaction between the origin recognition complex (ORC) and genomic DNA. In mammalian cells, this interaction appears to be regulated by Orc1, the only ORC subunit that contains a bromo-adjacent homology (BAH) domain. Since BAH domains mediate protein-protein interactions, the human Orc1 BAH domain was mutated, and the mutant proteins expressed in human cells to determine their affects on ORC function. The BAH domain was not required for nuclear localization of Orc1, association of Orc1 with other ORC subunits, or selective degradation of Orc1 during S-phase. It did, however, facilitate reassociation of Orc1 with chromosomes during the M to G1-phase transition, and it was required for binding Orc1 to the Epstein-Barr virus oriP and stimulating oriP-dependent plasmid DNA replication. Moreover, the BAH domain affected Orc1's ability to promote binding of Orc2 to chromatin as cells exit mitosis. Thus, the BAH domain in human Orc1 facilitates its ability to activate replication origins in vivo by promoting association of ORC with chromatin.

Original languageEnglish
Pages (from-to)5372-5382
Number of pages11
JournalEMBO Journal
Issue number22
Publication statusPublished - 2006 Nov 15
Externally publishedYes


  • Cell cycle
  • DNA replication
  • Epstein-Barr virus
  • Origin recognition complex
  • oriP

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology


Dive into the research topics of 'The BAH domain facilitates the ability of human Orc1 protein to activate replication origins in vivo'. Together they form a unique fingerprint.

Cite this