The detergent-insoluble microdomains, rafts, can be used as an effective immunogen

Yohko U. Katagiri, Hideki Nakajima, Ban Sato, Yoshitaka Miyagawa, Yasuomi Horiuchi, Hajime Okita, Junichiro Fujimoto, Nobutaka Kiyokawa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Detergent-insoluble microdomains, or rafts, act as a platform to transduce signals from the extracellular space into the cytoplasm. In the process of developing monoclonal antibodies against raft molecules for the purpose of studying the molecular mechanism of raft-mediated signaling, we observed the uniqueness and certain advantages of immunization with rafts. Simple subcutaneous injection of mice with a phosphate-buffered saline (PBS) suspension of rafts without mixing with Freund's adjuvant made it possible to increase the titer of antiserum reacting with raft components. Interestingly, injection of rafts prepared from certain specific cell lines induced monoglycolipid-specific antibodies. Furthermore, antibodies were produced by raft-immunization of even syngeneic mice. Our findings suggest that this phenomenon does not represent a breakdown of immunological self-tolerance, but typical immune reactions accompanying the class switch from IgM antibodies to IgG antibodies.

Original languageEnglish
Pages (from-to)495-501
Number of pages7
JournalGlycoconjugate Journal
Issue number6
Publication statusPublished - 2008 Aug
Externally publishedYes


  • Antibody
  • Immune Response
  • Monoglycolipid-specific
  • Raft
  • Syngeneic antigen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'The detergent-insoluble microdomains, rafts, can be used as an effective immunogen'. Together they form a unique fingerprint.

Cite this