TY - GEN
T1 - The effect of temperature on the morphology of fibroblasts in a collagen gel in nutrient-free medium
AU - Yamada, Hiroshi
AU - Nobuhara, Shinji
AU - Miyata, Shogo
PY - 2008/9/22
Y1 - 2008/9/22
N2 - The effect of temperature on the morphology of fibroblasts in a collagen gel (living dermal model (Toyobo, Japan)) was investigated to And an efficient way to delay the occurrence of pressure ulcers in malnourished patients. Cells were observed microscopically after culture for 0, 3, and 6 h at 37 or 25°C in Dulbecco's phosphate-buffered saline (DPBS) in an incubator under 5% CO 2/95% air and 100% humidity. We measured the aspect ratio of cells located 10 μm below the gel surface after 0, 3, and 6 h under each condition. The mean±SD aspect ratio was 16.9±5.1 and 7.1±4.0 (n = 16) at 0 and 6 h, respectively, for cells incubated at 37°C and 13.8±4.1 and 14.5±6.1 (n = 16) at 0 and 6 h, for cells incubated at 25°C. This indicates that cell viability can be maintained by lowering the temperature when the supply of nutrients is limited.
AB - The effect of temperature on the morphology of fibroblasts in a collagen gel (living dermal model (Toyobo, Japan)) was investigated to And an efficient way to delay the occurrence of pressure ulcers in malnourished patients. Cells were observed microscopically after culture for 0, 3, and 6 h at 37 or 25°C in Dulbecco's phosphate-buffered saline (DPBS) in an incubator under 5% CO 2/95% air and 100% humidity. We measured the aspect ratio of cells located 10 μm below the gel surface after 0, 3, and 6 h under each condition. The mean±SD aspect ratio was 16.9±5.1 and 7.1±4.0 (n = 16) at 0 and 6 h, respectively, for cells incubated at 37°C and 13.8±4.1 and 14.5±6.1 (n = 16) at 0 and 6 h, for cells incubated at 25°C. This indicates that cell viability can be maintained by lowering the temperature when the supply of nutrients is limited.
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U2 - 10.1109/ITAB.2008.4570592
DO - 10.1109/ITAB.2008.4570592
M3 - Conference contribution
AN - SCOPUS:51849168664
SN - 9781424422555
T3 - 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
SP - 539
EP - 540
BT - 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
T2 - 5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
Y2 - 30 May 2008 through 31 May 2008
ER -
