We evaluated the effects of Kampo-medicines (Sho-saiko-to, Keishi-bukuryo-gann and Mao-bushi-saishin-to), or various mitogens (concanavalin A, anti-CD 3 monoclonal antibody and lipopolysaccharide) on drug-induced lymphocyte stimulation test (DLST). BALB/c mice were administered orally with Sho-saiko-to (SST) or vehicle (Control). In Control splenocytes, Sho-saiko-to showed mitogenic activity, and the constituent herb Kanzo had the strongest activity. In SST splenocytes, cytokine production by anti-CD 3 and mitogenic response by all mitogenes and medicines were enhanced. Some modulations in immune response were found also in SST hepatic mononuclear cells. The immunostimulatory activity of Sho-saiko-to, as a polyclonal mitogen in vitro and as an immunopotentiator in vivo, may confuse the diagnosis for side effects of Kampo-medicines using DLST.
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