The influence of serum for spreading of tumor cells on synthetic glycolipid films

Toshinori Sato, Masanori Endo, Yoshio Okahata

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Adhesion and spreading of tumor cells to the films of a galactose-, glucose-, or phosphatidylcholine-bearing lipid was studied. Human adenocarcinoma Hela cells, B16 mouse melanoma cells, and HuH-7 human hepatoma cells selectively adhered and spread on galactose-bearing lipid in serum-containing medium, but not in serum-free medium. The spreading of the tumor cells in serum-containing medium was inhibited in the presence of lactose, but not in the presence of maltose. Cell spreading also occured on the galactose-bearing glycolipid film pre-treated with serum. From quantitative analysis for the adsorption of serum components by a quartz-crystal microbalance, the surfaces of the lipid films were found to be entirely covered with serum components. These results suggested that serum components pre-adsorbed on the galactose-bearing lipid influence the cell spreading.

Original languageEnglish
Pages (from-to)587-599
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Issue number7
Publication statusPublished - 1996
Externally publishedYes


  • B16 mouse melanoma cell
  • Cell spreading
  • Fetal bovine serum
  • Glycolipid
  • Hela cell
  • HuH-7 human hepatoma cell
  • Phospholipid
  • Quartz-crystal microbalance

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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