The promoter of the endo A cytokeratin gene is activated by a 3′ downstream enhancer

Yoshihiro Takemoto, Yasuo Fujimura, Midori Matsumoto, Yoshitaka Tamai, Takashi Morita, Aizo Matsushiro, Masami Nozaki

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Mouse cytokeratin EndoA is an intermediate filament subunit of the type II cytokeratin class which initiates expression in trophectoderm cells of blastocyst during embryogenesis. To identify the regulatory elements of the endo A gene, we constructed a series of CAT expression vectors and transfected them into PYS-2 cells. We found an enhancer element locating 1 kb downstream from the endo A gene which acts on both the endo A and SV40 promoters. This enhancer consists of six direct repeated sequences with homology to the PEA3 motif in polyoma virus α enhancer core. In undifferentiated F9 embryonal carcinoma cells, expression of the construct containing the enhancer was not detected. These results indicate that one of the regulatory mechanisms of endo A gene expression is the 3′ downstream enhancer.

Original languageEnglish
Pages (from-to)2761-2765
Number of pages5
JournalNucleic acids research
Issue number10
Publication statusPublished - 1991 May 25
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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