The role of calcium in platelet microtubule assembly-disassembly

Y. Ikeda; M. Kikuchi; M. Handa; Y. Yoshii; K. Toyama; M. Yamamoto; K. Watanabe; Y. Ando

doi:10.1016/0049-3848(81)90248-6

The role of calcium in platelet microtubule assembly-disassembly

Y. Ikeda, M. Kikuchi, M. Handa, Y. Yoshii, K. Toyama, M. Yamamoto, K. Watanabe, Y. Ando

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The role of calcium in the equilibrium between assembled and disassembled microtubules was investigated in human platelets. The two pools of microtubule protein was "frozen" by addition of a glycerol-dimethyl sulfoxide-containing medium and were estimated by measuring the colchicine binding activities of total and polymerized tubulin. Addition of A23187 to platelet rich plasma produced a transient decrease in the pool of polymerized tubulin within 30 sec., followed by a return to base-line values within 2 min.. Total content of platelet tubulin remained unchanged during the course of aggregation. TMB-8, a known intracellular calcium antagonist, abolished this transient decrease in polymerized tubulin induced by A23187, while indomethacin did not. These findings strongly suggest the important role of intracellular calcium in microtubule assembly-disassembly.

Original language	English
Pages (from-to)	163-168
Number of pages	6
Journal	Thrombosis Research
Volume	23
Issue number	1-2
DOIs	https://doi.org/10.1016/0049-3848(81)90248-6
Publication status	Published - 1981
Externally published	Yes

Keywords

Microtubules
calcium ionophore
intracellular calcium
tubulin

ASJC Scopus subject areas

Hematology

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10.1016/0049-3848(81)90248-6

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title = "The role of calcium in platelet microtubule assembly-disassembly",

abstract = "The role of calcium in the equilibrium between assembled and disassembled microtubules was investigated in human platelets. The two pools of microtubule protein was {"}frozen{"} by addition of a glycerol-dimethyl sulfoxide-containing medium and were estimated by measuring the colchicine binding activities of total and polymerized tubulin. Addition of A23187 to platelet rich plasma produced a transient decrease in the pool of polymerized tubulin within 30 sec., followed by a return to base-line values within 2 min.. Total content of platelet tubulin remained unchanged during the course of aggregation. TMB-8, a known intracellular calcium antagonist, abolished this transient decrease in polymerized tubulin induced by A23187, while indomethacin did not. These findings strongly suggest the important role of intracellular calcium in microtubule assembly-disassembly.",

keywords = "Microtubules, calcium ionophore, intracellular calcium, tubulin",

author = "Y. Ikeda and M. Kikuchi and M. Handa and Y. Yoshii and K. Toyama and M. Yamamoto and K. Watanabe and Y. Ando",

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T1 - The role of calcium in platelet microtubule assembly-disassembly

AU - Ikeda, Y.

AU - Kikuchi, M.

AU - Handa, M.

AU - Yoshii, Y.

AU - Toyama, K.

AU - Yamamoto, M.

AU - Watanabe, K.

AU - Ando, Y.

PY - 1981

Y1 - 1981

N2 - The role of calcium in the equilibrium between assembled and disassembled microtubules was investigated in human platelets. The two pools of microtubule protein was "frozen" by addition of a glycerol-dimethyl sulfoxide-containing medium and were estimated by measuring the colchicine binding activities of total and polymerized tubulin. Addition of A23187 to platelet rich plasma produced a transient decrease in the pool of polymerized tubulin within 30 sec., followed by a return to base-line values within 2 min.. Total content of platelet tubulin remained unchanged during the course of aggregation. TMB-8, a known intracellular calcium antagonist, abolished this transient decrease in polymerized tubulin induced by A23187, while indomethacin did not. These findings strongly suggest the important role of intracellular calcium in microtubule assembly-disassembly.

AB - The role of calcium in the equilibrium between assembled and disassembled microtubules was investigated in human platelets. The two pools of microtubule protein was "frozen" by addition of a glycerol-dimethyl sulfoxide-containing medium and were estimated by measuring the colchicine binding activities of total and polymerized tubulin. Addition of A23187 to platelet rich plasma produced a transient decrease in the pool of polymerized tubulin within 30 sec., followed by a return to base-line values within 2 min.. Total content of platelet tubulin remained unchanged during the course of aggregation. TMB-8, a known intracellular calcium antagonist, abolished this transient decrease in polymerized tubulin induced by A23187, while indomethacin did not. These findings strongly suggest the important role of intracellular calcium in microtubule assembly-disassembly.

KW - Microtubules

KW - calcium ionophore

KW - intracellular calcium

KW - tubulin

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JO - Thrombosis Research

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IS - 1-2

ER -

The role of calcium in platelet microtubule assembly-disassembly

Abstract

Keywords

ASJC Scopus subject areas

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