The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer

Yoshihiko Sakurai; Yoshihide Otani; Kaori Kameyama; Naoki Igarashi; Tetsuro Kubota; Koichiro Kumai; Masaki Kitajima

doi:10.1002/(SICI)1096-9098(199711)66:3<168::AID-JSO3>3.0.CO;2-A

The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer

Yoshihiko Sakurai, Yoshihide Otani, Kaori Kameyama, Naoki Igarashi, Tetsuro Kubota, Koichiro Kumai, Masaki Kitajima

Division of Diagnostic Pathology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.

Original language	English
Pages (from-to)	168-172
Number of pages	5
Journal	Journal of Surgical Oncology
Volume	66
Issue number	3
DOIs	https://doi.org/10.1002/(SICI)1096-9098(199711)66:3<168::AID-JSO3>3.0.CO;2-A
Publication status	Published - 1997 Nov

Keywords

Collagenase
Fibroblast
Gastric cancer
Matrix metalloproteinase-1
Stromal cell

ASJC Scopus subject areas

Surgery
Oncology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/(SICI)1096-9098(199711)66:3<168::AID-JSO3>3.0.CO;2-A

Cite this

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title = "The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer",

abstract = "Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.",

keywords = "Collagenase, Fibroblast, Gastric cancer, Matrix metalloproteinase-1, Stromal cell",

author = "Yoshihiko Sakurai and Yoshihide Otani and Kaori Kameyama and Naoki Igarashi and Tetsuro Kubota and Koichiro Kumai and Masaki Kitajima",

year = "1997",

month = nov,

doi = "10.1002/(SICI)1096-9098(199711)66:3<168::AID-JSO3>3.0.CO;2-A",

language = "English",

volume = "66",

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journal = "Journal of Surgical Oncology",

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TY - JOUR

T1 - The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer

AU - Sakurai, Yoshihiko

AU - Otani, Yoshihide

AU - Kameyama, Kaori

AU - Igarashi, Naoki

AU - Kubota, Tetsuro

AU - Kumai, Koichiro

AU - Kitajima, Masaki

PY - 1997/11

Y1 - 1997/11

N2 - Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.

AB - Background and Objectives: In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods: We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results: Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions: These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells.

KW - Collagenase

KW - Fibroblast

KW - Gastric cancer

KW - Matrix metalloproteinase-1

KW - Stromal cell

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The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase-1) in the invasion of gastric cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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