TY - JOUR
T1 - The sialyl-Tn antigen synthase genes regulates migration–proliferation dichotomy in prostate cancer cells under hypoxia
AU - Yamamoto, Daiki
AU - Hongo, Hiroshi
AU - Kosaka, Takeo
AU - Aoki, Natsumi
AU - Oya, Mototsugu
AU - Sato, Toshinori
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023/4
Y1 - 2023/4
N2 - A low-oxygen (hypoxia) tumor microenvironment can facilitate chemotherapy and radiation therapy resistance in tumors and is associated with a poor prognosis. Hypoxia also affects PCa (prostate cancer) phenotype transformation and causes therapeutic resistance. Although O-glycans are known to be involved in the malignancy of various cancers under hypoxia, the expression and function of O-glycans in PCa are not well understood. In this study, the saccharide primer method was employed to analyze O-glycan expression in PCa cells. Results showed that the expression of sTn antigens was increased in PCa cells under hypoxia. Furthermore, it was found that ST6GalNAc1, the sTn antigen synthase gene, was involved in the migration–proliferation dichotomy and drug resistance in PCa cells under hypoxia. The results of this study will contribute to the development of novel diagnostic markers and drug targets for PCa under hypoxia.
AB - A low-oxygen (hypoxia) tumor microenvironment can facilitate chemotherapy and radiation therapy resistance in tumors and is associated with a poor prognosis. Hypoxia also affects PCa (prostate cancer) phenotype transformation and causes therapeutic resistance. Although O-glycans are known to be involved in the malignancy of various cancers under hypoxia, the expression and function of O-glycans in PCa are not well understood. In this study, the saccharide primer method was employed to analyze O-glycan expression in PCa cells. Results showed that the expression of sTn antigens was increased in PCa cells under hypoxia. Furthermore, it was found that ST6GalNAc1, the sTn antigen synthase gene, was involved in the migration–proliferation dichotomy and drug resistance in PCa cells under hypoxia. The results of this study will contribute to the development of novel diagnostic markers and drug targets for PCa under hypoxia.
KW - Hypoxia
KW - LC-MS
KW - Migration-proliferation dichotomy
KW - O-glycan
KW - Prostate cancer
KW - Saccharide primer method
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U2 - 10.1007/s10719-023-10104-z
DO - 10.1007/s10719-023-10104-z
M3 - Article
C2 - 36806956
AN - SCOPUS:85148359696
SN - 0282-0080
VL - 40
SP - 199
EP - 212
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
IS - 2
ER -
