TY - JOUR
T1 - The Xenopus IP3 receptor
T2 - Structure, function, and localization in oocytes and eggs
AU - Kume, Shoen
AU - Muto, Akira
AU - Aruga, Jun
AU - Nakagawa, Toshiyuki
AU - Michikawa, Takayuki
AU - Furuichi, Teiichi
AU - Nakade, Shinji
AU - Okano, Hideyuki
AU - Mikoshiba, Katsuhiko
N1 - Funding Information:
H. 0. is the corresponding author. We thank Dr. T. lnoue for computer analysis of DNA sequences, Dr. H. Kawame for technical assistance, Drs. S. Sakata and D. W. Cleveland for generous supplies of expression vectors, Dr. G. Kuwajima for a monoclonal antibody to RyR, and Dr. D. A. Melton for a cDNA library of Xenopus oocytes. We also thank Drs. R. Moerschell, A. Miyawaki, M. Yuzaki, and N. Sagatafor critical comments on the manuscript. We are grateful to members of the Mi-koshiba Laboratory for valuable discussions. This work was supported by a grant for Specially Promoted Research to K. M.; grants from the Japanese Ministry of Education, Science, and Culture to T. F., J. A., and H. 0.; and a grant from the Yamanouchi on Metabolic Disorders.
PY - 1993/5/7
Y1 - 1993/5/7
N2 - To study the role of the IP3 receptor (IP3R) upon egg activation, cDNA clones encoding IP3R expressed in the Xenopus oocytes were isolated. By analyses of the primary structure and functional expression of the cDNA, Xenopus IP3R (XIP3R) was shown to have an IP3-binding domain and a putative Ca2+ channel region. Immunocytochemical studies revealed polarized distribution of XIP3R in the cytoplasm of the animal hemisphere in a well-organized endoplasmic reticulum-like structure and intensive localization in the perinuclear region of stage VI immature oocytes. In ovulated unfertilized eggs, XIP3R was densely enriched in the cortical region of both hemispheres in addition to its polarized localization. After fertilization, XIP3R showed a drastic change in its distribution in the cortical region. These results imply the predominant role of the XIP3R in both the formation and propagation of Ca2+ waves at fertilization.
AB - To study the role of the IP3 receptor (IP3R) upon egg activation, cDNA clones encoding IP3R expressed in the Xenopus oocytes were isolated. By analyses of the primary structure and functional expression of the cDNA, Xenopus IP3R (XIP3R) was shown to have an IP3-binding domain and a putative Ca2+ channel region. Immunocytochemical studies revealed polarized distribution of XIP3R in the cytoplasm of the animal hemisphere in a well-organized endoplasmic reticulum-like structure and intensive localization in the perinuclear region of stage VI immature oocytes. In ovulated unfertilized eggs, XIP3R was densely enriched in the cortical region of both hemispheres in addition to its polarized localization. After fertilization, XIP3R showed a drastic change in its distribution in the cortical region. These results imply the predominant role of the XIP3R in both the formation and propagation of Ca2+ waves at fertilization.
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U2 - 10.1016/0092-8674(93)90142-D
DO - 10.1016/0092-8674(93)90142-D
M3 - Article
C2 - 8387895
AN - SCOPUS:0027335486
SN - 0092-8674
VL - 73
SP - 555
EP - 570
JO - Cell
JF - Cell
IS - 3
ER -