Thermoresponsive anionic block copolymer brushes with a strongly anionic bottom segment for effective interactions with biomolecules

Kenichi Nagase; Jun Kobayashi; Akihiko Kikuchi; Yoshikatsu Akiyama; Hideko Kanazawa; Teruo Okano

doi:10.1039/c6ra20944k

Thermoresponsive anionic block copolymer brushes with a strongly anionic bottom segment for effective interactions with biomolecules

Kenichi Nagase, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Teruo Okano

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Brushes were prepared from a thermoresponsive strongly anionic block copolymer, namely poly(2-acrylamido-2-methylpropanesulfonic acid) (AMPS)-b-poly(N-isopropylacrylamide) (PAMPS-b-PIPAAm), by multistep atom-transfer radical polymerization with pH control of the reaction solution. The prepared block copolymer brushes were characterized using CHN elemental analysis, X-ray photoelectron spectroscopy, contact angle measurements, and zeta potential measurements. The results showed that dense polymer brushes were formed on the silica surfaces, and showed thermally modulated changes in their hydrophobic and anionic properties. Chromatographic analyses using silica beads modified with PAMPS-b-PIPAAm brushes as column-packing materials showed that the block copolymer brushes interacted more strongly with basic biomolecules than did brushes of a random copolymer, namely P(IPAAm-co-AMPS). PAMPS-b-PIPAAm copolymer brushes could therefore provide effective thermoresponsive anionic interfaces with strong anionic properties that could be modulated by changing the external temperature.

Original language	English
Pages (from-to)	93169-93179
Number of pages	11
Journal	RSC Advances
Volume	6
Issue number	95
DOIs	https://doi.org/10.1039/c6ra20944k
Publication status	Published - 2016

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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title = "Thermoresponsive anionic block copolymer brushes with a strongly anionic bottom segment for effective interactions with biomolecules",

abstract = "Brushes were prepared from a thermoresponsive strongly anionic block copolymer, namely poly(2-acrylamido-2-methylpropanesulfonic acid) (AMPS)-b-poly(N-isopropylacrylamide) (PAMPS-b-PIPAAm), by multistep atom-transfer radical polymerization with pH control of the reaction solution. The prepared block copolymer brushes were characterized using CHN elemental analysis, X-ray photoelectron spectroscopy, contact angle measurements, and zeta potential measurements. The results showed that dense polymer brushes were formed on the silica surfaces, and showed thermally modulated changes in their hydrophobic and anionic properties. Chromatographic analyses using silica beads modified with PAMPS-b-PIPAAm brushes as column-packing materials showed that the block copolymer brushes interacted more strongly with basic biomolecules than did brushes of a random copolymer, namely P(IPAAm-co-AMPS). PAMPS-b-PIPAAm copolymer brushes could therefore provide effective thermoresponsive anionic interfaces with strong anionic properties that could be modulated by changing the external temperature.",

author = "Kenichi Nagase and Jun Kobayashi and Akihiko Kikuchi and Yoshikatsu Akiyama and Hideko Kanazawa and Teruo Okano",

note = "Funding Information: This research was partly financially supported by the Development of New Environmental Technology Using Nanotechnology Project of the National Institute of Environmental Science (NIES), commissioned by the Ministry of the Environment, Japan; Grants-in-Aid for Scientific Research (C) No. 26420714 from the Japan Society for the Promotion of Science (JSPS), Japan; and subsidies from the Kumagai Foundation for Science and Technology. Publisher Copyright: {\textcopyright} 2016 The Royal Society of Chemistry. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2016",

doi = "10.1039/c6ra20944k",

language = "English",

volume = "6",

pages = "93169--93179",

journal = "RSC Advances",

issn = "2046-2069",

publisher = "Royal Society of Chemistry",

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T1 - Thermoresponsive anionic block copolymer brushes with a strongly anionic bottom segment for effective interactions with biomolecules

AU - Nagase, Kenichi

AU - Kobayashi, Jun

AU - Kikuchi, Akihiko

AU - Akiyama, Yoshikatsu

AU - Kanazawa, Hideko

AU - Okano, Teruo

N1 - Funding Information: This research was partly financially supported by the Development of New Environmental Technology Using Nanotechnology Project of the National Institute of Environmental Science (NIES), commissioned by the Ministry of the Environment, Japan; Grants-in-Aid for Scientific Research (C) No. 26420714 from the Japan Society for the Promotion of Science (JSPS), Japan; and subsidies from the Kumagai Foundation for Science and Technology. Publisher Copyright: © 2016 The Royal Society of Chemistry. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2016

Y1 - 2016

N2 - Brushes were prepared from a thermoresponsive strongly anionic block copolymer, namely poly(2-acrylamido-2-methylpropanesulfonic acid) (AMPS)-b-poly(N-isopropylacrylamide) (PAMPS-b-PIPAAm), by multistep atom-transfer radical polymerization with pH control of the reaction solution. The prepared block copolymer brushes were characterized using CHN elemental analysis, X-ray photoelectron spectroscopy, contact angle measurements, and zeta potential measurements. The results showed that dense polymer brushes were formed on the silica surfaces, and showed thermally modulated changes in their hydrophobic and anionic properties. Chromatographic analyses using silica beads modified with PAMPS-b-PIPAAm brushes as column-packing materials showed that the block copolymer brushes interacted more strongly with basic biomolecules than did brushes of a random copolymer, namely P(IPAAm-co-AMPS). PAMPS-b-PIPAAm copolymer brushes could therefore provide effective thermoresponsive anionic interfaces with strong anionic properties that could be modulated by changing the external temperature.

AB - Brushes were prepared from a thermoresponsive strongly anionic block copolymer, namely poly(2-acrylamido-2-methylpropanesulfonic acid) (AMPS)-b-poly(N-isopropylacrylamide) (PAMPS-b-PIPAAm), by multistep atom-transfer radical polymerization with pH control of the reaction solution. The prepared block copolymer brushes were characterized using CHN elemental analysis, X-ray photoelectron spectroscopy, contact angle measurements, and zeta potential measurements. The results showed that dense polymer brushes were formed on the silica surfaces, and showed thermally modulated changes in their hydrophobic and anionic properties. Chromatographic analyses using silica beads modified with PAMPS-b-PIPAAm brushes as column-packing materials showed that the block copolymer brushes interacted more strongly with basic biomolecules than did brushes of a random copolymer, namely P(IPAAm-co-AMPS). PAMPS-b-PIPAAm copolymer brushes could therefore provide effective thermoresponsive anionic interfaces with strong anionic properties that could be modulated by changing the external temperature.

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Thermoresponsive anionic block copolymer brushes with a strongly anionic bottom segment for effective interactions with biomolecules

Abstract

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