Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment

Aya Mizutani; Akihiko Kikuchi; Jun Kobayashi; Yoshikatsu Akiyama; Hideko Kanazawa; Teruo Okano

Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment

Aya Mizutani, Akihiko Kikuchi, Jun Kobayashi, Yoshikatsu Akiyama, Hideko Kanazawa, Teruo Okano

School of Pharmaceutical Sciences

Research output: Contribution to conference › Paper › peer-review

Abstract

In the present research, thermoresponsive interfaces grafted with high-density poly(N-isopropylacrylamide) (PIPAAm) are prepared by surface-initiated atom transfer radical polymerization (ATRP) on poly(p-chloromethylstyrene)-cast polystyrene surfaces. Surface characterization revealed that thermoresponsive polymers were covalently grafted onto the polystyrene surfaces. Moreover, we investigated influence of thermoresponsive surface characteristics on adhesion/detachment behavior of cultured cells. Results suggest that control of the amount of grafted PIPAAm, and also the differences in its PIPAAm constructed structure influence cell adhesion/detachment control.

Original language	English
Pages	5399-5400
Number of pages	2
Publication status	Published - 2006 Dec 1
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 2006 Sept 20 → 2006 Sept 22

Other

Other	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	06/9/20 → 06/9/22

Keywords

Cell culture
High-density polymer grafted surfaces
Poly(N-isopropylacrylamide)
Surface-initiated atom transfer radical polymerization
Thermoresponsive polymer

ASJC Scopus subject areas

Engineering(all)

Cite this

Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment. / Mizutani, Aya; Kikuchi, Akihiko; Kobayashi, Jun et al.
2006. 5399-5400 Paper presented at 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, Japan.

Research output: Contribution to conference › Paper › peer-review

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title = "Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment",

abstract = "In the present research, thermoresponsive interfaces grafted with high-density poly(N-isopropylacrylamide) (PIPAAm) are prepared by surface-initiated atom transfer radical polymerization (ATRP) on poly(p-chloromethylstyrene)-cast polystyrene surfaces. Surface characterization revealed that thermoresponsive polymers were covalently grafted onto the polystyrene surfaces. Moreover, we investigated influence of thermoresponsive surface characteristics on adhesion/detachment behavior of cultured cells. Results suggest that control of the amount of grafted PIPAAm, and also the differences in its PIPAAm constructed structure influence cell adhesion/detachment control.",

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year = "2006",

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T1 - Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment

AU - Mizutani, Aya

AU - Kikuchi, Akihiko

AU - Kobayashi, Jun

AU - Akiyama, Yoshikatsu

AU - Kanazawa, Hideko

AU - Okano, Teruo

PY - 2006/12/1

Y1 - 2006/12/1

N2 - In the present research, thermoresponsive interfaces grafted with high-density poly(N-isopropylacrylamide) (PIPAAm) are prepared by surface-initiated atom transfer radical polymerization (ATRP) on poly(p-chloromethylstyrene)-cast polystyrene surfaces. Surface characterization revealed that thermoresponsive polymers were covalently grafted onto the polystyrene surfaces. Moreover, we investigated influence of thermoresponsive surface characteristics on adhesion/detachment behavior of cultured cells. Results suggest that control of the amount of grafted PIPAAm, and also the differences in its PIPAAm constructed structure influence cell adhesion/detachment control.

AB - In the present research, thermoresponsive interfaces grafted with high-density poly(N-isopropylacrylamide) (PIPAAm) are prepared by surface-initiated atom transfer radical polymerization (ATRP) on poly(p-chloromethylstyrene)-cast polystyrene surfaces. Surface characterization revealed that thermoresponsive polymers were covalently grafted onto the polystyrene surfaces. Moreover, we investigated influence of thermoresponsive surface characteristics on adhesion/detachment behavior of cultured cells. Results suggest that control of the amount of grafted PIPAAm, and also the differences in its PIPAAm constructed structure influence cell adhesion/detachment control.

KW - Cell culture

KW - High-density polymer grafted surfaces

KW - Poly(N-isopropylacrylamide)

KW - Surface-initiated atom transfer radical polymerization

KW - Thermoresponsive polymer

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M3 - Paper

AN - SCOPUS:33846161290

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T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment

Abstract

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Keywords

ASJC Scopus subject areas

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